苜蓿遗传多样性的取样数目——RAPD和SSR群体标记法  

Sampling Numbers in Genetic Diversity Analysis of Alfalfa Using Bulked DNA Based on RAPD and SSR Markers

作　　者：班霆[1] 韩鹏[1] 刘翔[1] 金樑[1] 王晓娟[1] 

机构地区：[1]兰州大学草地农业科技学院,中国甘肃兰州730020

出　　处：《生命科学研究》2009年第2期158-162,共5页Life Science Research

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB108904);甘肃省自然科学基金资助项目(3ZS051-A25-066)

摘　　要：紫花苜蓿为异花授粉植物,其DNA多态性研究的取样策略与遗传多样性分析直接相关.选取了4个苜蓿品种陇东(地方品种)、中兰1号(育成品种)、牧歌(Graze)和金皇后(Queen)(引进品种),设置了取样数目分别为10、20、40和60个单株进行DNA混合,利用RAPD(random amplification polymorphic DNA,随机扩增长度多态性DNA)和SSR(simple sequence repeat,简单重复序列)标记分别进行了遗传多样性分析,结果发现40和60个单株DNA混合样的聚类结果一致,表明10和20个单株组成的群体太小,随着苜蓿群体取样数目的增加,遗传多样性分析的准确性也随之增加,但是分析成本也相应提高.鉴于此,利用RAPD和SSR标记分析苜蓿遗传多样性时采用40个单株的DNA混合样是较适宜的群体大小.Alfalfa （Medicago sativa L.） belongs to the typical allogamy, and the sampling strategies are closely related to its molecular genetic diversity analysis. Four alfalfa varieties, Lngdong （local variety）, Zhonglan 1 （synthetic variety）, Graze and Queen （introduced variety）, were selected and their bulked DNA samples obtained from 10, 20, 40 and 60 individual plants, respectively. The results of unweighted pair-group method with arithmetic averages （UPGMA） analysis based on random amplification polymorphic DNA （RAPD） and simple sequence repeat （SSR） markers showed that 40 and 60 bulked DNA sample methods had the same genetic relationships between these 4 alfalfa varieties, which indicated that populations with 10 and 20 individual plants were too small, and the accurate analysis were positive related to the sampling numbers but the cost of analysis increased also. According to the results above, 40 individual plants was the suitable sampling strategies in genetic diversity analysis of alfalfa based on RAPD and SSR markers.

关 键 词：紫花苜蓿 RAPD SSR 群体标记法 

分 类 号：S815[农业科学—畜牧学] Q943[农业科学—畜牧兽医]