褪黑素减轻STZ大鼠高血糖及胰腺组织内氧化应激水平的研究  

Study of Melatonin on Releasing Hyperglycemia and the Severity of Oxidative Stress in Pancreatic Tissues of Streptozotocin Rats

作　　者：王怀清[1,2] 雷银雪[2] 刘敏 苏明丽[2] 邹俊杰[1] 刘志民[1] 

机构地区：[1]第二军医大学上海长征医院内分泌科,上海200003 [2]湖北省宜昌市第一人民医院内分泌科,宜昌443000 [3]湖北省宜昌市中医院内二科,宜昌443000

出　　处：《中国医药导刊》2009年第1期81-82,共2页Chinese Journal of Medicinal Guide

基　　金：国家"九七三"资助项目(2005CB523304)

摘　　要：目的:研究链脲佐菌素(streptozotocin , STZ)对大鼠血糖及胰腺组织内氧化应激影响以及褪黑素( melatonin, MLT)的保护作用。方法:大鼠随机分为三组,正常对照组(NC) , STZ组(STZ) ,褪黑素+STZ组(MLT+STZ),各10只大鼠。STZ(70 mg/kg)一次性腹腔内注射以诱发糖尿病,注射前8天开始给予MLT (200ug/kg/day,强饲)直至实验结束(4周)。实验开始及结束时测动物血糖,称体重各一次,实验结束时处死动物,取胰腺组织行生化检测。结果:STZ使大鼠血糖明显升高,同时升高胰腺组织内MDA水平,降低胰腺组织内GSH水平及GSH-Px活性;MLT预处理降低STZ大鼠的血糖及胰腺组织内MDA水平,增加GSH水平及GSH-Px活性。结论:MLT通过减轻胰腺组织内氧化应激水平保护胰岛β细胞功能的完整性。Objective： To investigate the protective effects of melatonin （MLT） against hyperglycemia and the severity of oxidative stress in pancreatic tissues induced by streptozotocin （STZ ） in rats.Methods： Thirty rats were randomly divided into three groups： Normal control （NC） group,STZ group and MLT﹢STZ group.MLT （200ug /kg/day,oral gavage） was given for 8 days prior to induction of diabetes with STZ （70 mg/kg,i.p.） until the end of the study （4 weeks）;Blood glucose levels and biomarkers of oxidative stress in pancreatic homogenates were measured.Results： STZ induced a significant increase in blood glucose levels and malonaldehyde （MDA） levels in pancreatic homogenates,a significant decrease in glutathione （GSH） levels and glutathione peroxidase （GSH-Px） activities.Pretreatment of STZ rats with MLT decreased blood glucose levels,MDA levels and increased GSH levels and GSH-Px activities in pancreatic homogenates.Conclusion： MLT exerts its protective effect against hyperglycemia through reducing the severity of oxidative stress in pancreatic tissues.

关 键 词：褪黑素 链脲佐菌素 糖尿病 动物模型 高血糖 氧化应激 胰岛β细胞功能 

分 类 号：R965[医药卫生—药理学]