肿瘤坏死因子-α和骨形成发生蛋白_2诱导成纤维细胞成骨表型表达的体外研究  

REGULATORY FACTORS OF OSTEOGENIC PHENOTYPICAL EXPERESSION BY FIBROBLASTS IN VITRO

作　　者：邓廉夫[1,2] 汤雪明[1,2] 柴本甫[1,2] 李慧[1,2] 

机构地区：[1]上海第二医科大学细胞生物学实验室 [2]上海市伤骨科研究所

出　　处：《中国修复重建外科杂志》1998年第4期236-240,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金

摘　　要：成纤维细胞的成骨作用已在体内外得到证实。为进一步探索诱导成纤维细胞成骨潜能得以表现的调控因素，以实现体外获取大量成骨型成纤维细胞、形成骨修复材料的设想，采用分离、纯化人皮肤成纤维细胞，使其生长在含不同浓度的ＥＧＦ、ＩＬ６、ＴＮＦα、ＢＭＰ２培养液的干预条件下，采用生物化学、组织化学和电镜观察方法，检测成纤维细胞成骨性标志物形成状况。发现：ＴＮＦα和ＢＭＰ２联合应用，可使成纤维细胞分泌碱性磷酸酶、骨钙素及胶原的量增加；成纤维细胞由梭形向圆形或多突形转化，蛋白分泌旺盛；细胞外基质中，丰富的胶原纤丝定向或杂乱排列，其间散在较多的钙颗粒；成纤维细胞可重叠交织形成多层结构，其表面分泌颗粒和钙盐结晶堆积，并不断融合扩大成骨结节。认为。In order to explore further the regulatory factors to the potentiality in inducing osteogenesis by fibroblasts, the fibroblasts were isolated, and purified from human skin, and were grown in incubation in the media of EGF, IL6, TNFα and BMP2 at different concentrations for two weeks, then, the markers for osteogenic features were investigated by biochemistry, histochemistry and electron microscopic observations. It was found that the combined use of TNFα and BMP2 could stimulate fibroblasts to secrete alkaline phosphatase, osteocalcin and collagen, and the morphological changes of the fibroblasts were also very stroking. In the extracellular matrix, the collagen fibrils, with or without periodicity, were arranged regularly or randomly oriented, and numerous minute calcium granules were interspersed among them. The fibroblasts were interwoven one on top of another in the form of multilayer structure and on the surface, there were secreting granules and piling up of calcium crystals which coalessed steadily and increased in size in forming bony nodules. It was considered that TNFα and BMP2 were capable of inducing the fibroblasts to form bone.

关 键 词：成纤维细胞 成骨 肿瘤坏死因子 骨形成蛋白 

分 类 号：R329-34[医药卫生—人体解剖和组织胚胎学] R68[医药卫生—基础医学]