反复冻融及超深低温处理的异体肌腱移植实验研究  

EXPERIMENTAL STUDY OF HOMOGRAFT OF REPEATED FREEZINGTHAWING TREATED AND ULTRALOWTEMPERATURE TREATED TENDON

作　　者：唐林俊[1,2] 程国良[1,2] 方光荣 陈秉礼[1,2] 杨志贤 

机构地区：[1]解放军第401医院全军显微外科中心手外科 [2]重庆医科大学附属第二医院骨科

出　　处：《中国修复重建外科杂志》1998年第4期241-245,共5页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：为比较有创与无创冷冻方法处理的肌腱异体移植的效果，探索不同冷冻处理技术对肌腱免疫源性的影响及获得成功的异体移植的条件，采用有创反复冻融及无创超深低温（－１９６℃）冻存技术分别处理肌腱，液氮下保存３个月后异体移植，以自体肌腱移植作对照。腱细胞活力测定显示，反复冻融处理的肌腱细胞全部失活，而超深低温处理的肌腱细胞活力为（９２．５±３．４）％。组织学观察显示，反复冻融及超深低温处理的异体移植肌腱均产生了不同程度炎性细胞浸润，且腱周粘连均较自体移植肌腱重。主动屈曲功能测定、羟脯氨酸含量测定及生物力学性能测定显示，反复冻融与超深低温组无显著性差异（Ｐ＞０．０５），且后两项指标显著较自体移植肌腱差。认为：①反复冻融及超深低温处理的肌腱异体移植获得一定成功，两者无显著性意义。②既要保留肌腱细胞的活性，又要去除肌腱中的抗原呈递细胞，可能是冷冻处理的肌腱异体移植获得成功的关键。③损伤了肌腱细胞成分，降低了肌腱抗原性。In order to compare the immunogenecity and biological properties of homologous tendon grafts after treatment from different methods of freezing, tendons from chickens received repeated freezingthawing treatment or ultralowtemperature treatment, and then, the posttreatment tendons were preserved in liquid nitrogen for 3 months before transplantation. The autogenous tendon transplantation was served as the control. It was found that in the group of repeated freezingthawing treated tendons, the tendon cells all died and while in the ultralow temperature treated tendons the active rate of tendon cells was 92.5%±3.4%, and the histological observation showed that transplantation of frozen tendons would result in extensive infiltration of inflammatory cells in the grafted tendons and the peritendinous adhesion was serious than that of the autografts. The active flexion function, hydroxyproline levels and the biomechanical analysis showed no significant differences between the repeated freezingthawing treated homografts and the ultralowtemperature treated homografts, and that the autografts was definitely superior to the homografts. The conclusions were: (1) Transplantation of the homologous tendons from the two different methods of freezing could receive considerable success and there was no significant difference between them; (2) Transplantation of frozen homologous tendon graft might give successful result which was probably due to the preservation of the cellular activity of the tendon cells following freezing treatment and elimination of the antigen presenting cells in the tendon as well, and (3) Although the cellular components of the tendon were damaged and the antigenicity of the tendon was lowered, it did not necessarily mean that homologous tendon graft would always be successful in transplantation.

关 键 词：肌腱移植 异体移植 冷冻 低温处理 动物实验 

分 类 号：R622.2[医药卫生—整形外科] R687.2[医药卫生—外科学]