pGE.GmIL-12/脂质体对变应性鼻炎小鼠嗜酸性粒细胞的影响  

Effect of intranasal pGEG.mIL-12/lipoplex on the eosinophils in the murine model of allergic rhinitis

作　　者：韩锦华[1] 王彤[2] 周兵[2] 范尔钟[2,3] 鞠淑英[2] 孙桂秋[1] 

机构地区：[1]大连大学附属新华医院耳鼻咽喉科,辽宁大连116021 [2]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京100730 [3]北京市耳鼻咽喉科研究所,北京100005

出　　处：《中国耳鼻咽喉头颈外科》2009年第3期154-157,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的探讨鼻腔局部应用脂质体包裹的EB病毒(epstein-barr virus,EBV)质粒载体介导白细胞介素12(interleukin-12,IL-12)基因治疗对变应性鼻炎小鼠模型鼻黏膜、外周血和骨髓中嗜酸性粒细胞(eosinophils,Eos)的调节作用。方法采用6～8周雄性BALB/C小鼠,随机分成变应性鼻炎组、基因治疗组和正常对照组,每组各12只。以卵清蛋白致敏激发建立变应性鼻炎组模型,治疗组用阳离子脂质体包裹EBV质粒载体介导的IL-12表达质粒(pGEG.mIL-12)形成混合物EBV/lipoplex,于激发前鼻腔局部滴入。分别用HE染色计数鼻黏膜中Eos数量,用瑞氏染色计数骨髓涂片中Eos比例,以流式细胞仪检测外周血中Eos占粒细胞比例。结果小鼠基因治疗组鼻黏膜Eos显著低于变应性鼻炎模型(P<0.01);骨髓涂片中Eos比例显著低于变应性鼻炎模型(P<0.01);基因治疗组外周血中Eos显著低于变应性鼻炎模型(P<0.05)。结论经鼻局部给予脂质体包裹的pGEG.mIL-12能够减少骨髓、外周血和鼻黏膜中Eos数量,可能为呼吸道变应性炎症开辟新的治疗方法。OBJECTIVE To evaluate the effect of intranasal liposome-mediated IL-12 gene therapy on the eosinophils in the murine model of allergic rhinitis. METHODS Thirty-six BALB/C mice were randomly divided into allergic rhinitis （AR） group, gene therapy group and control group. Allergic rhinitis group were sensitized and suscitated ovalb umin （ovalbumin, OVA） , and gene therapy group were administered with liposome-mediated pGEG.mlL-12 per nasal before suscitation. The eosinophils in bone marrow were counted by Wright＇s staining, and the eosinophils in nasal mucosa were counted by H-E staining. The eosinophils of peripheral blood were detected by flow cytometry. RESULTS The ratio of eosinophils to white cells in nasal mucosa and bone marrow of gene therapy group was significantly lower than that of AR group （P〈0.01） .The ratio ofeosinophils to granulocyte in peripheral blood were significantly lower in gene therapy group than in AR group （P〈0.05） . CONCLUSION Administration of liposomemediated pGEG.mlL-12 per nasal could depress the proliferation and differentiation of eosinophils and decrease the delivery and transference of eosinophils to peripheral blood and nasal mucosa. It probably develops a new treatment for respiratory tract allergic inflammation.

关 键 词：鼻炎 变应性 常年性 白细胞介素12 基因 抑制 嗜酸细胞 脂质体 

分 类 号：R765.21[医药卫生—耳鼻咽喉科] R562.25[医药卫生—临床医学]