ELISA双抗原夹心法检测鼠疫FI抗体的效果评价  

Effective evaluation on detecting plague FI antibody by DAgS-ELISA

作　　者：王海峰[1] 史献明[1] 杜国义[1] 董国润[1] 杨顺林[1] 张懿辉[1] 刘合智[1] 

机构地区：[1]河北省鼠疫防治所,张家口075000

出　　处：《中国地方病防治》2009年第2期112-113,共2页Chinese Journal of Control of Endemic Diseases

摘　　要：目的比较ELISA双抗原夹心法与IHA法检测人血清中鼠疫FI抗体的最低检出限,符合率以及检测ELISA的特异性,以确定ELISA检测技术能否在鼠疫监测、诊断和流行病学调查中得到应用。方法分别用ELISA双抗原夹心法和IHA(间接血凝法)检测血清298份和鼠疫阳性诊断血清1份。结果ELISA双抗原夹心法检出4份阳性血清,IHA也检出4份阳性血清,2种方法阳性检出率无差别,符合率为75%,最低检出限均为1∶160。结论ELISA特异性强,阳性检出率与IHA相当,结果容易判定,可以在鼠疫病例诊断及鼠疫监测中作为常规方法推广使用。Objective To compare the minimum and accord of DAgS - ELISA （ double antigens sandwich Enzymelinked immunosorbent assay） with IHA （ indirect hemagglutination assay ） detecting plague FI antibody in human serum,to detective the specific and to evaluate effects of DAgS- ELISA in the plague serodiagnosis,surveillance and epidemiological investigation. Methods 298 samples of serum collected from Kangbao county and Li county,and 1 sample of positive serum were detected by DAgS - ELISA and IHA . Results 4 positive serum were found out using both DAgS - ELISA and IHA. The positive rate of two methods is the same. The minimum of detecting is 1：160. The accord is 75%. Conclusions DAgS - ELISA is specific and easier to judge. The positive rate of ELISA and IHA was similar. DAgS - ELISA could be used in diagnosis of human and animal plague.

关 键 词：ELISA 鼠疫 FI抗体 效果评价 

分 类 号：R516.8[医药卫生—内科学]