IL-1β抑制精氨酸升压素促心脏成纤维细胞胶原凝胶的收缩  

Effects of IL-1β on collagen gel contraction induced by argipressin in cardiac fibroblasts

作　　者：彭育红[1] 赵连友[2] 汝磊生[1] 陈永清[2] 范延红[2] 杨学东[2] 

机构地区：[1]解放军白求恩国际和平医院心内科,河北石家庄050082 [2]第四军医大学唐都医院心内科,陕西西安710038

出　　处：《心脏杂志》2009年第2期161-164,共4页Chinese Heart Journal

摘　　要：目的探讨白细胞介素-1β（IL-1β）对精氨酸升压素（AVP）诱导下心脏成纤维细胞（cF）胶原凝胶收缩的作用。方法以胰蛋白酶消化法分离、培养SD大鼠的CF，建立成纤维细胞与胶原构成的三维培养体系，测定基础状态下不同浓度的IL-1β作用下，CF胶原凝胶收缩指数，以及IL-1β对AVP作用后cF胶原凝胶收缩指数的影响。实验共分为8个组，即不同浓度（0、50、100、200及400kU／L）的IL-1β组、不同浓度（0.1×10^-7mol／L）的AVP干预组及100kU／L IL-1β＋1×10^-7mol／LAVP组，每组各设8个孔（n=8）。结果在基础状态下，以50～400kU／L IL-1β作用后，cF胶原凝胶收缩指数随着IL-1β干预浓度的增加呈下降趋势，各实验组胶原凝胶收缩指数均明显低于对照组（P〈0．01），各实验组之间两两比较的差异也非常显著（P〈0．01）。1×10^-7mol／LAVP组cF胶原凝胶收缩指数明显高于0mol／LAVP组（P〈0．01）；而100kU／L IL-1β组CF胶原凝胶收缩指数明显低于0kU／L IL-1组（P〈0．01）。IL-1β与AVP共同作用后，CF胶原凝胶收缩指数介于AVP组和IL-1β组之间，并且与二者之间的差异均有非常显著的意义（P〈0．01）。结论IL-1β可抑制基础状态下CF胶原凝胶收缩，并对AVP促cF胶原凝胶收缩具有明显的拈抗作用。AIM To explore the effects of interleukin （IL）-1β on collagen gel contraction induced by argipressin （AVP） in cardiac fibroblasts （CF）. METHODS Using trypsin separate and culture cardiac fibroblasts （CF） of SD neonatal rats, we set up a three-dimensional culture system of CF and collagen gel. RESULTS With the pretreatment with IL-1β （50 -400 kU/L） in CF, the collagen gel contraction index decreased with the increasing concentration of IL-1β, and the index in each test group was signifi- cantly lower than that in control （ P 〈 0.01 ）. The collagen gel contraction index of pretreatment with AVP was significantly higher than that in control （ P 〈 0. 01 ） whereas the index of pretreatment with IL-1β was significantly lower than that in control （P 〈 0.01 ）. Following simultaneous pretreatment with AVP and IL-1β, the index was between those of the two groups, with significant difference between AVP or IL-1β （P 〈0.01 ）. CONCLUSION IL-1β inhibits eollagen gel contraction in basic condition and antagonizes the effects of AVP on collagen gel contraction.

关 键 词：白细胞介素-1Β 精氨酸升压素 心脏成纤维细胞 胶原凝胶收缩 

分 类 号：R392.12[医药卫生—免疫学]