BIOMED-2系统引物用于检测T细胞淋巴瘤石蜡样本T细胞受体γ基因重排的研究  

Application of BIOMED-2 primers in analysis of T-cell receptor γ gene rearrangements in paraffin-embedded tissue specimens of T-cell lymphoma

作　　者：唐源[1] 江炜[2] 李雷[2] 纪洪[3] 李韵[1] 李甘地[1] 刘卫平[1] 

机构地区：[1]四川大学华西医院病理科,成都610041 [2]华西第二医院病理科 [3]山东省立医院病理科

出　　处：《中华病理学杂志》2009年第4期253-257,共5页Chinese Journal of Pathology

摘　　要：目的了解BIOMED-2系统T细胞受体（TCR）γ引物组合对T细胞淋巴瘤的常规石蜡包埋组织样本中TCR基因重排的检出情况及其实用性。方法用酚／氯仿法提取55例各种组织类型的T细胞淋巴瘤石蜡包埋组织样本的DNA并通过扩增看家基因β-globin检测其质量，利用BIOMED-2系统TCRγ引物组合和TCRγ基因通用型引物（TVG／TJX）对55例进行TCR基因重排检测，比较二者的检出率并进行统计学分析。结果BIOMED-2系统TCRγ引物组合和TCRγ基因通用型引物（TVG／TJX）的TCR基因重排检出率分别为76．4％和60．0％，前者高于后者，二者的差异无统计学意义（P〉0．05）。结论BIOMED-2系统TCR7引物组合适用于本组T细胞淋巴瘤石蜡包埋组织样本的TCR基因重排检测。Objective To evaluate the practical values of PCR detectable T-cell receptor （TCR） gene rearrangement in paraffin embedded tissue samples in the diagnosis of T-cell malignancies using BIOMED-2 PCR multiplex tubes TCRγ（ A ＋ B）. Methods Traditional phenol-chloroform method was used to extract DNA from 55 cases of archival paraffin embedded tissues samples of T-cell malignancies and the DNA quality was evaluated by PCR-based amplification of housekeeping gene β-globin. The selected BIOMED-2 PCR multiplex tubes TCRγ（ A ＋ B） were used to detect TCR gene rearrangement and comparison with the results of universal TCR primers （TVG/TJX） was performed. Results Positive detection rates by the BIOMED-2 multiplex tubes TCRγ（ A ＋ B ） and the universal primers TVC/TJX） were 76. 4% and 60. 0%, respectively. There were not statistical difference between the methods （ P 〉 0. 05 ）. Conclusion BIOMED-2 multiplex tubes TCRγ（ A ＋ B） is suitable for detection of clonal rearrangements of TCR genes in current archival paraffin embedded tissue samples of T-cell malignancies.

关 键 词：淋巴瘤 T细胞 受体 抗原 T细胞 基因重排 聚合酶链反应 石蜡包埋 组织细胞学制备技术 BIOMED-2系统 

分 类 号：R686[医药卫生—骨科学]