人牙周韧带细胞成骨分化的差异表达蛋白质组学研究  

A differential expression proteomic study of human periodontal ligament cell during osteogenic differentiation

作　　者：吴莉萍[1] 韦曦[1] 凌均棨[1] 刘路[1] 

机构地区：[1]中山大学光华口腔医学院牙体牙髓科,广州510055

出　　处：《中华口腔医学杂志》2009年第4期212-216,共5页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30872876）;广东省科技计划项目（2008B0301075）

摘　　要：目的获得人牙周韧带细胞诱导成骨分化通路中的调节蛋白，探讨牙周韧带细胞诱导成骨分化的分子机制。方法应用胶内差异双向电泳结合基质辅助激光解吸电离一飞行时间质谱鉴定等蛋白质组学技术筛选人牙周韧带细胞诱导成骨分化的差异表达蛋白质组。结果获得人牙周韧带细胞诱导成骨分化7d的差异表达蛋白质图谱，确认有显著差异的蛋白质斑点61个，质谱鉴定29个蛋白质，包括细胞骨架蛋白及细胞骨架相关蛋白、膜结合蛋白、核蛋白、基质合成、代谢酶和信号传导等蛋白。其中一组细胞骨架蛋白及细胞骨架相关蛋白同时发生改变，与牙周韧带细胞成骨分化早期细胞骨架重组、有丝分裂和细胞迁移等过程紧密相关。结论建立了人牙周韧带细胞成骨分化早期的差异蛋白质组图谱，为牙周韧带细胞成骨分化的蛋白调控机制进一步研究提供了实验依据。Objective To obtain regulating proteins during human periodontal ligament cell （hPDLC） osteogenic differentiation and investigate its underlying molecular mechanism. Methods Twodimension difference in gel electrophoresis （2D-DIGE） coupled with matrix-assissted laser adsorption ionization-time of flight mass spectrometry （MALDI-TOF-MS） analysis was used to identify regulating proteins during hPDLC osteogenie differentiation. Results A differently expressed protein profile was obtained 7 days after osteogenic induction, and 61 protein spots with significant differences, 29 protein spots were identified by MALDI-TOF-MS, including cytoskeleton, cell membrane-bounding, nuclear regulation, matrix synthesis, and metabolic enzymes and signal transduction and other proteins. A functional class of cytoskeleton proteins showed co-regulation, these proteins were intimately involved in the reorganization of the cytoskeleton, cytokinesis and migration during PDLC differentiation. Conclusions A database of differently displayed proteins during hPDLC osteogenic differentiation was established, which may be helpful to understand and further study molecular mechanism of PDLC osteogenic differentiation.

关 键 词：蛋白质组学 电泳 凝胶 双向 人牙周韧带细胞 成骨分化 

分 类 号：R686[医药卫生—骨科学]