运用原子力显微术磁驱动模式研究活软骨细胞形貌超微结构和力学特性  

Research of topographic ultra-structure and biomechanical properties of living chondrocytes cells with atomic force microscopy

作　　者：刘广源[1] 吴志宏[1] 陈佩佩[2] 李兵[1] 谢琳[2] 韩东[2] 邱贵兴[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院骨科,100730 [2]国家纳米科学中心纳米医学与生物实验室

出　　处：《中华医学杂志》2009年第13期912-915,共4页National Medical Journal of China

摘　　要：目的运用原子力显微镜（AFM）最新发展的磁驱动轻敲模式（MAC mode）研究体外培养的正常人软骨细胞表面形貌的结构特点及细胞力学特性。方法体外培养正常人膝关节软骨细胞，运用MAC mode AFM在生理条件下对细胞表面形貌进行高分辨成像，并通过指定区域的力曲线操作，观察软骨细胞核区及胞质区的力学特性。结果MAC mode AFM可实现生理条件下软骨细胞的表面形貌超微结构的高分辨成像，分辨率可达30nm。软骨细胞呈现核区突出，胞质区平坦的形貌特点。同时，胞质区与核区对比表现出更高的应力状态及黏着力。结论MAC mode AFM是研究活细胞微观结构和力学特性的有力工具。Objective To evaluate the application of a novel tapping mode, magnetic AC mode （MAC mode） of atomic force microscopy （AFM） in observation of the topography and mechanical properties of living chondrocytes. Methods Specimens of normal knee joint was obtained from .5 patients during amputation due to severe trauma of shank. Chondrocytes were isolated and cultured. Cell slides were prepared, underwent immunohistoehemieal staining, and observed under AFM by MAC mode. Force curve operation was performed at different regions, nuclear and cytoplsamic, to compare the topographic features. Results High-resolution images of the ultra-structure of cell surface were obtained under physiological condition with a resolution level to 30 nm. The cells presented a topical profile, i. e. , the protuberant nuclear region and relatively flatten cell body. The force curve showed much higher stress and adhesion in the cell body region than in the nuclear region. Conclusion MAC mode AFM is very useful in research of the ultra-structure and biomechanical properties of cells.

关 键 词：显微镜检查 原子力 软骨细胞 生物力学 

分 类 号：R686[医药卫生—骨科学]