生物检材中阿维菌素的HPLC-MS/MS分析  

Determination of avermectins in biological human samples by HPLC-MS/MS

作　　者：董颖[1] 王炯[1] 何毅[1] 

机构地区：[1]公安部物证鉴定中心,北京100038

出　　处：《中国法医学杂志》2009年第2期96-98,共3页Chinese Journal of Forensic Medicine

摘　　要：目的建立生物检材包括血、肝组织、胃组织中阿维菌素(Avermectins)的HPLC-MS/MS分析方法。方法采用Oasis HLB固相萃取柱进行提取,以XTerraTM RP18柱(2.1mm×100mm,3.5μm)色谱柱分离,以甲醇-0.1%冰醋酸水溶液(75∶25)为流动相,流速为0.2mL/min。结果线性范围10ng/mL～3μg/mL,最小检出限为0.1ng/mL。结论本方法准确、快速,可用于生物检材血中阿维菌素的定性定量分析,肝及胃组织中阿维菌素的定性分析。Objective To establish HPLC-MS/MS method for the determination of avermeetins in biological Human Samples. Methods Samples were extracted by method of solid-phase extraction. XTerra^TM RP18 column （2.1 mm × 100 mm, 3.5 μm） and the mobile phase with methanol：acetic acid of 0. 1% （75 ： 25） were used. The flow rate was 0. 2 mL · min ^- 1 Results There was a good linear relationship within the range of 10 ng/mL -3 μg/mL. The limit of detection was 0.1 ng/mL . Conclusions The method is simple, rapid, accurate . It can be used for the determination of avermectins in biological human samples.

关 键 词：法医毒物分析 阿维菌素 生物检材 固相萃取 HPLC-MS/MS 

分 类 号：D919[医药卫生—法医学]