纳米氧化锌对人胚肺成纤维细胞的生物毒性  

Cytotoxicity on Human Embryonic Lung Fibroblasts of ZnO Nanoparticles in Cosmetics

作　　者：袁金华[1] 李光[1] 陈慧珍[2] 查河霞[1] 宋黎军[1] 

机构地区：[1]南京市疾病预防控制中心理化科,江苏南京210003 [2]中山大学东校区教学实验中心,广东广州510006

出　　处：《中山大学学报（医学科学版）》2009年第2期170-173,178,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：南京市医学重点科技发展项目(ZKX06030);江苏省预防医学科研课题(Y2006026)

摘　　要：【目的】研究化妆品添加剂-氧化锌纳米粒子对正常人胚肺成纤维细胞(HELF)的生物毒性。【方法】采用MTT法检测氧化锌纳米粒子对体细胞存活率的影响;利用光学显微镜评价细胞的形态学变化;电子显微镜观察细胞与纳米氧化锌作用前后表面微结构的变化。【结果】在测试的2.5～150mg·L-1浓度范围内,氧化锌纳米粒子对HELF细胞均有抑制作用,细胞毒性呈明显的剂量依赖效应关系。浓度在20mg·L-1以上时,氧化锌纳米粒子可致使细胞生存率低于10%。荧光显微镜可见典型的细胞凋亡改变。扫描电子显微镜观察到HELF细胞表面与高浓度纳米粒子作用后产生的纳米级孔。【结论】纳米氧化锌粒子由于小尺寸效应和Zn2+自身毒性的协同作用,高浓度时抑制细胞结活性,致使细胞死亡。在化妆品中添加氧化锌纳米粒子作为紫外屏蔽剂时,建议控制浓度为20mg·L-1以下。旨在对化妆品生产企业在功效添加方面起到警示作用,也为卫生监督提供理论依据。[ Objective ] To study the potential biological toxicity of nanosized ZnO particles used as cosmetic additives on human embryonic lung fibroblasts （HELF） cells. [Methods] The effect of ZnO nanoparticles on the survival rate of normal cell line HELF was evaluated by MTT assay. Morphological changes of these cells were evaluated by optical microscope and the surface microstructure of cells before and after nanosized ZnO treatment were observed by scanning electric microscopy （SEM）. [ Results ] At the concentration of 2.5 - 150 mg ·L^-1, ZnO nanoparticles water suspensions had inhibitory effect on HELF cells and the cytotoxicity showed an obvious dose-dependent relationship. While the concentrations were more than 20 mg ·L^-1, ZnO nanoparticles led to the livability of the tested cells lower than 10%. HELF cells apoptosis could be observed under fluorescence microscope. Furthermore, SEM images showed nanometer holes in HELF cells surface induced by high-concentration nanoparticles. [Conclusions] Due to the small size effect and synergistic effect of their toxicity, high-concentration ZnO nanoparticles lead to the inhibition of cell viability and cell death. Therefore, it is suggested that the concentration should be less than 20 mg ·L^-1 when ZnO nanoparticles were used as the auti-UV agents in cosmetics for the sake of people health. It is to provide some caution in additives in cosmetics industrial enterprise and theoretical basis for health supervision.

关 键 词：肺 成纤维细胞细胞 氧化锌纳米粒子 生物毒性 化妆品添加剂 

分 类 号：R114[医药卫生—卫生毒理学]