HPLC法测定芷鹅鼻炎颗粒中野黄芩苷的含量  

Determination of Scutellarin in Zhi-e Rhinitis Granules With HPLC Method

作　　者：饶伟源[1] 兰保强[1] 李茂[1] 覃潇[1] 

机构地区：[1]广西中医药研究院,广西南宁530022

出　　处：《中医药导报》2009年第3期74-75,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立芷鹅鼻炎颗粒中野黄芩苷的含量测定方法。方法:采用HPLC法,色谱柱:kromasil C18柱(4.6 mm×250mm,5μm);流动相:甲醇-水-醋酸(46∶54∶1);流速:1.0 mL/min;检测波长:335 nm。结果:野黄芩苷在0.0802-2.0060μg范围内线性关系良好,r=0.9996,平均回收率为98.90%,RSD%=1.09%。结论:本法简便,可靠,重现性好,可作为本品的质量控制指标。Objective： To establish a method for determination of scutellarin in Zhi-e rhinitis granules. Methods： HPLC method was used, and consisted of kromasil C18 chromatographic column（4.6 mm×250 mm,5 μm） with mobile phase of methanolwater-acetic acid（46：54：1） at the detection wavelength of 335 nm, the flow rate was 1.0mL/min. Results： Scutellarin showed a linear relationship at the range of 0.0802-2.0060 μg, r=0.9996, the average recovery was. 98.0% with RSD% of 1.09%. Conclusion： The method is simple, relible with good reproducibility, can be used as quality control for this product.

关 键 词：芷鹅鼻炎颗粒 HPLC 野黄芩苷 

分 类 号：R927.2[医药卫生—药学]