波纹唇鱼不同组织5种同工酶表达的差异  

Five kinds of isozyme in different tissues of Cheilinus undulatus

作　　者：区又君[1] 齐旭东[1,2] 李加儿[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]广东海洋大学,广东湛江524025

出　　处：《南方水产》2009年第2期51-55,共5页South China Fisheries Science

基　　金：国家高技术研究发展计划(863计划)项目(2006AA10A414)

摘　　要：运用聚丙烯酰胺垂直梯度凝胶电泳法对波纹唇鱼(Cheilinus undulatus)6组织(肌肉、心脏、肝脏、肾脏、脑和脾脏)的5种同工酶[酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)和天门冬氨酸氨基转移酶(AST)]进行了研究,并对其同工酶位点及其酶谱表型进行了分析。结果表明,波纹唇鱼5种同工酶系统具有不同程度的组织特异性。EST检测到2条酶带,由2个基因座位编码。LDH在6种组织中都存在,共检测到9条酶带,由3个基因座位编码,具有多态性现象。MDH在6种组织中均可检测到,可分为线粒体型和上清液型,各由1个基因座位编码,组织差异性不显著。ME检测到2条酶带,肝脏的含量比较丰富,线粒体型和上清液型的区分不明显。AST在6种组织中都没有发现。Five isozymes （EST, ME, AST, LDH and MDH） in six tissues （muscle, heart, liver, kidney, brain and spleen） of Cheilinus undulatus were studied by using vertical polyacrylamide gel gradient electrophoresis. The results showed that the five isozymes presented tissues specificity to some degrees. The number of EST bands was 2, which were encoded by 2 loci. LDH existed in all tissues studied. The number of LDH bands was 9, which were encoded by 3 loci, and the polymorphism was strong. Two types of MDH （m-MDH and s-MDH） were found and also existed in the six tissues. The number of ME bands was 2. ME in liver were rich, but it was not distinct between m-ME and s-ME. There was no AST in all six tissues.

关 键 词：波纹唇鱼 同工酶 表达 组织特异性 

分 类 号：Q55[生物学—生物化学]