HPLC法测定复方毛冬青注射液中腺苷的含量  

Content determination of Ribosidoadenine in Fufang Maodongqing Injection by HPLC

作　　者：冯达星 邓六勤[2] 陈怡禄[2] 

机构地区：[1]广州市白云区第二人民医院,广东广州510450 [2]广州市儿童医院,广东广州510120

出　　处：《中国医药导报》2009年第10期68-69,共2页China Medical Herald

基　　金：广州市卫生局资助项目(2005A027;30307-3200516)

摘　　要：目的:建立复方毛冬青注射液中腺苷含量的高效液相测定方法。方法:采用HPLC法测定腺苷的含量。色谱柱为Kromasil C18柱(4.6mm×250mm,5μm),流动相为磷酸盐缓冲液(pH6.5)-甲醇(17∶3),柱温为室温,流速为1ml/min,检测波长为260nm。结果:腺苷在0.18～0.90μg范围内与其峰面积呈良好的线性关系(r=0.9999,n=5),平均回收率为99.58%(RSD=0.42%)。结论:该测定方法精密度高,准确性、重现性好,可用于复方毛冬青注射液的质量控制。Objective： To establish a determination method of Ribosidoadenine in Fufang Maodongqing Injection by HPLC. Methods： The determination was carried out by HPLC with a Kromasil C18（4.6 min×250 mm, 5μm） column, the mobile phase was phosphate buffered solution-Methanol（17：3） with a flow rate of 1 ml/min, detection wavelength was 260 nm. Results：Ribosidoadenine showed a good linear relationship in the range of 0.18-0.90μg （r=-0.999 9, n=5）, the average recovery was 99.58%（RSD=0.42%）. Conclusion： The method is sensitive, accurate, reproducible, specific and can be used for quality control of Fufang Maodongqing Injection.

关 键 词：复方毛冬青注射液 腺苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]