大黄对糖尿病大鼠肾脏ICAM-1及VCAM-1表达的影响  

Influence of Rhubarb on Renal Expression of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 in Diabetes Mellitus Rats

作　　者：田风胜[1] 李文东[1] 段洪波[1] 苏秀海[1] 王晓蕴[1] 

机构地区：[1]河北医科大学附属沧州中西医结合医院内分泌科 ,沧州061001

出　　处：《国际中医中药杂志》2009年第2期105-106,共2页International Journal of Traditional Chinese Medicine

摘　　要：目的探讨大黄对糖尿病大鼠肾脏细胞间黏附分子-1（intercellular adhesion molecular-1，ICAM-1）及血管细胞间黏附分子-1（Vascular CellAdhesion Molecule-1，VCAM-1）表达的影响。方法制备糖尿病大鼠模型，成模后，随机抽取24只分为模型组、大黄组各12只。大黄组给予大黄煎剂10g（生药·kg体重^-1）灌胃，正常组和模型组给予同等剂量纯净水灌胃。8周后，取肾脏制备病理切片，SP法免疫组化染色，观察ICAM-1、VCAM-1的表达。结果大黄组、模型组ICAM—1及VCAM—1表达明显高正常组（P〈0．05），大黄组肾脏ICAM-1、VCAM-1表达弱于模型组（P〈0.05）。结论大黄能抑制糖尿病大鼠肾脏ICAM-1、VCAM-1表达（P〈0．05），具有保护糖尿病大鼠肾脏的作用。Objective To explore the influence of DaHuang on renal expression of intercellular adhesion molecule- 1 （ICAM- 1） and vascular cell adhesion molecule- 1 （VCAM-1） in diabetes mellitus rats. Methods After the DM rat model was made, 24 DM rats were selected randomly and divided into a model group （12 DM rats） and a Rhubarb group （12 DM rats）. Rhubarb was given to rhubarb group with 10g · kg ^-1 through mouth per day. The model group and the normal group were given pure water in equal amount every day. 8 weeks later, kidney was cut to make pathological slice and method of SP immunohistochemistry was adopted for staining. Observed the expression of ICAM-1 and VCAM-1. Result The renal expression of ICAM-t and VCAM-I in the rhubarb group and the model group were obviously increased compared with the normal group （P〈0.05）. The renal expression of ICAM-1 and VCAM-1 in the rhubarb group was obviously weaker than the normal group（P〈0.05）. Conclusion Rhubarb could obviously inhibit the renal expression of 1CAM-1 and VCAM-1 in diabetes mellitus rats and protect kidney of diabetes mellitus rats.

关 键 词：大黄 糖尿病模型大鼠 肾脏细胞间黏附分子-1 血管细胞间黏附分子-1 

分 类 号：R285.5[医药卫生—中药学]