东北马鹿Myostatin基因单核苷酸多态性研究  

Single Nucleotide Polymorphism Analysis of Myostatin Gene in Northeast Wapiti

作　　者：巴恒星[1] 邢秀梅[1] 刘晓颖[1] 张秀华[1] 苗利光[1] 杨福合[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2009年第1期11-13,18,共4页Special Wild Economic Animal and Plant Research

基　　金：科技部特种经济动物高效养殖技术研究(2006AD06B06)

摘　　要：Myostatin基因即肌肉生长抑制素,是一种肌肉生长的负调控因子。以物种最近的牛(Bos taurus cattle登陆号:AB076403)的Myostatin基因序列为模版,根据该基因保守序列,进行引物设计。应用pcr-sscp和测序的方法对东北马鹿Myostatin基因的第3外显子进行了多态性分析。结果表明,位于Myostatin基因DNA序列第3外显子948处发生G→A突变。该突变并没有引起氨基酸的变异,GTA、GTG密码子都编码缬氨酸(val)。Myostatin is a negative regulator of skeletal muscle growth. Myostatin gene sequence of Notheast Wapiti was cloned based on the closest species of cattle （Bos taurus cattle accession： AB076403） for the templates, with primer＇s design according to the conservative region of MSTN gene. Using a pair of special primers to amplify the exon 3 of Myostatin gene in Northeast Wapiti, we aimed to detect the polymorphisms of Myostatin gene. We found that it was G mutated to A at 948 point in Myostatin gene cDNA sequence in deer. The mutation does not cause amino acid variations, GTA,GTG codon codes valine （val）.

关 键 词：东北马鹿 MYOSTATIN基因 PCR-SSCP 

分 类 号：S825[农业科学—畜牧学] Q349.55[农业科学—畜牧兽医]