反相高效液相色谱法测定利巴韦林注射液的有关物质  

Determination of Related Substances in Ribavirin Injection by RP-HPLC

作　　者：吴宏富[1] 刘放[2] 

机构地区：[1]浙江省庆元县中医院,浙江庆元323800 [2]浙江省医学科学院药物研究所,浙江杭州310013

出　　处：《中国药业》2009年第7期17-18,共2页China Pharmaceuticals

摘　　要：目的建立测定利巴韦林注射液有关物质的反相高效液相色谱(RP-HPLC)法。方法采用Hypersil C18色谱柱(250mm×4.6mm,10μm),以水为流动相,检测波长为207nm。结果利巴韦林及其有关物质达到基线分离,方法的最低检测量为2.5ng。结论方法简便、快速、准确,适用于利巴韦林及其制剂的质量控制。Objective To establish a RP- HPLC method for the determination of relevant substances in Ribavirin Injection. Methods The RP- HPLC method was used. Chromatographic condition included the Hypersil C18 column with the mobile phase of water. The UV detection wavelength was set at 207 nm. Results The peak of ribavirin and its related substances were separated completely. LOD was 2.5 ng. Conclusion The method is simple, rapid and accurate, which can be used to control the quality of ribavirin and its preparations.

关 键 词：利巴韦林 利巴韦林注射液 有关物质 反相高效液相色谱法 

分 类 号：R978.7[医药卫生—药品] R927.11[医药卫生—药学]