抗β1-肾上腺素受体抗体对大鼠T淋巴细胞的作用  

The effects of antibody against β1-adrenaline receptor on T lymphocytes

作　　者：包海军[1] 白克华[2] 王瑾[1] 左琳[1] 马秀瑞[1] 宋峰[1] 燕子[1] 贺忠梅[1] 刘慧荣[3] 

机构地区：[1]山西医科大学生理教研室山西医科大学细胞生理学省部共建教育部重点实验室,太原市030001 [2]山西医科大学药学院,太原市030001 [3]首都医科大学心脏病学系

出　　处：《中国心血管病研究》2009年第4期298-301,共4页Chinese Journal of Cardiovascular Research

基　　金：国家自然科学基金（30670835）;太原市2008年大学生创新创业专项项目（08122059）

摘　　要：目的观察抗β1-肾上腺素受体（AR）抗体对大鼠T淋巴细胞的作用。方法用人工合成的β1-肾上腺素受体细胞外第二环肽段（β1-AR—ECⅡ）作为抗原主动免疫大鼠，采用流式细胞技术测定大鼠外周血T淋巴细胞亚群的变化；观察抗β1-AR自身抗体对培养的淋巴结T细胞的影响。结果免疫组血清中T淋巴细胞亚群CD4^＋／CD8^＋在处理24h后开始升高，在第7天时达到高峰并持续2个月；抗β1-AR自身抗体可促进T淋巴细胞增殖，与β1-AR特异性阻断剂Metoprolol或β1-AR—ECⅡ共同作用时，可使抗体的促增殖效能显著减弱。结论抗β1-AR自身抗体可以使免疫系统失衡，促进T淋巴细胞的增殖。Objective To investigate the effects of antibody directed against the β1-adrenergic receptor （β1-AR） on T lymphocytes. Methods Wister rats were immunized with the synthetic peptide corresponding to the sequence of the second extracellular loop of β1-AR. The change of T lymphocyte subgroup in rat＇s peripheral blood was measured by flow cytometry. The effect of antibody directed against β1-AR on T lymphocytes was observed. Results Ratio of peripheric blood T lymphocyte immunophenotype reached peak value at 7 days in immunized groups and kept a higher level until 2 months after treatment, β1-AR autoantibodies could enhance the proliferation response of T lymphoeytes from rat. Metoprolol could attenuate the proliferation response induced by β1-AR- AA. Conclusion β1-AR autoantibodies could break the balance of immune system, and could promote proliferation of T lymphocytes from rats.

关 键 词：Β1-肾上腺素受体 自身抗体 T淋巴细胞 

分 类 号：Q95-33[生物学—动物学]