赖型钩体毒力相关基因ompL17的克隆表达及产物的体内分布  

CLONING, EXPRESSION OF THE VIRULENT-ASSOCIATED GENE OMPL17 OF LEPTOSPI-RA INTERROGENS SEROVAR LAI AND THE DISTRIBUTION OF THE EXPRESSION PRO-DUCTION IN VIVO

作　　者：张会东[1] 郝牧[2] 鲍朗[1] 张英[1] 黄毕[1] 高蕾[1] 

机构地区：[1]四川大学华西基础医学与法医学院感染免疫研究室,成都610041 [2]中国医学科学院血液病研究所重点实验室

出　　处：《现代预防医学》2009年第7期1331-1334,共4页Modern Preventive Medicine

基　　金：国家自然科学基金项目(30771908)

摘　　要：[目的]观察ompL17基因表达产物的免疫原性以及该表达蛋白在动物模型中的分布情况。[方法]提取钩端螺旋体017株基因组DNA,扩增出ompL17基因,与表达载体pGEX-4T-1重组,诱导表达OMPL17蛋白,免疫动物获得相应抗体,通过免疫组织化学技术观察该蛋白在动物模型组织当中的分布。[结果]PCR和酶切分析证实构建成功了表达载体,SDS-PAGE和Western-blot分析证实诱导表达出OMPL17蛋白,通过免疫组织化学技术观察到了该蛋白在动物模型中的分布情况。[结论]成功表达出ompL17基因的蛋白,观察到豚鼠动物模型中该蛋白的分布情况,为赖型钩体的分子机制的阐明奠定了基础。[Objective ] To observe the immunogenie and the distribution in animal model of the expression production of the gene ompL17. [Methods] The gene ompL17 was PCR-amplified from the genome DNA template of Leptospha interrogens strain 017.The cloned gene was ligated to plasmid pGEX-4T-1 and induced producing. The antibody was acquired from the immuned animal, and the distribution was observed in the animal model by immunohistochemistry. [ Results ] PCR, and restrietion endonuclease map revealed the successful construction of the gene expression vector and SDS-PAGE, Western-blot certified the successful expression of the gene ompL17.The expression production distribution was observed in the animal model by immunohistoehemistry. [Conclusion] The result of this study lays foundation for exploring molecule pathogenesis of Leptospira interrogens serovar lai.

关 键 词：钩端螺旋体 克隆 表达 体内分布 

分 类 号：R377.5[医药卫生—病原生物学]