应用4种方法检测狂犬病毒抗原的结果分析  

Analysis on the Detection of Rabies Virus by four Methods

作　　者：沈蕊华[1] 胡家瑜[1] 陆菁[1] 吕新军[2] 陶晓燕[2] 唐青[2] 

机构地区：[1]上海市疾病预防控制中心,上海200336 [2]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《海峡预防医学杂志》2009年第1期6-8,共3页Strait Journal of Preventive Medicine

摘　　要：[目的]分析上海市咬人犬的狂犬病毒检测方法,为狂犬伤及多人的公共突发性事件的处理和狂犬病监测提供检测依据。[方法]采集2003至2005年9月疑似狂犬脑标本230份,用ELISA法、直接免疫荧光法(dFA)、小鼠感染法(MIT)和巢式聚合酶链反应(巢式PCR)检测标本。[结果]4种方法结果一致:狂犬病毒阳性率21.3%(49/230)。阳性率以2004年为高(22.2%)。时间分布以春夏季为多。地区分布依次为嘉定、宝山和青浦等地。[结论]dFA、巢式-PCR和ELISA的两两组合可作为快速诊断的方法和狂犬病应急事件处理和预防控制的依据。[Objective] To analyze the detection methods of rabies virus for dealing with public emergency events and rabies inspection in Shanghai. [Methods] Totally 230 brain samples of suspect rabies dogs from 2003 to 2005 were collected and the virus antigen was detected by rapid ELISA, direct immuno-fluorescence assay （dFA）, mouse infection method and nest RT-PCR method. [Results] The results by 4 methods were identical： positive rate was 21.3% （49/ 230）. Positive rate was highest in 2004 （22.2 %）. The rate was higher in spring and summer. District distribution in order was Jiading, Baoshan and Qingpu et al. [Conclusions] dFA and ELISA or dFA and nest PCR or ELISA and nest PCR, every two methods for the detection of rabies virus can be used for rapid diagnosis of rabies virus, and for dealing with public emergency events and rabies control and prevention.

关 键 词：狂犬病 狂犬病毒 疾病控制 

分 类 号：R373.9[医药卫生—病原生物学]