两种方法检测乙型肝炎病毒表面抗原的比较  

Comparison of two methods in detecting HBsAg

作　　者：陈佑明[1] 黄敬[1] 刘京平[1] 李洁茹[1] 

机构地区：[1]南方医科大学附属华瑞医院检验科,广州510630

出　　处：《检验医学与临床》2009年第7期495-496,共2页Laboratory Medicine and Clinic

摘　　要：目的对胶体金免疫层析(GICA)法与酶联免疫吸附试验(ELISA)法检测乙型肝炎病毒表面抗原(HBsAg)进行比较。方法采用两种方法检测18～65岁人群血清标本4086份。结果GICA法检测出HBsAg阳性标本327份,阳性率为8.0%;ELISA法检测出阳性标本391份,阳性率为9.6%;GICA法与ELISA法检测标本符合率为98.43%。结论GICA法操作简便、快速,检测的特异度较好,但其灵敏度有限,ELISA法更适合医院、血站的检测应用。Objective To compare gold immunochromatography assay （GICA） with enzyme linked immunosorbent assay （ELISA） in detecting HBsAg. Methods GICA and ELISA were used to detect HBsAg in serum from 4 086 individuals aged from 18 to 65 years. Results A total of 327 samples （8.0%） were HBsAg positive when detected by GICA, while 391 cases （9.6%） showed positive when detected by ELISA; the positive coincidence rate was 98.43%. Conclusion Although GICA is simpler and faster than ELISA, and has a good specificity, its sensibili- ty is still dissatisfactory. By comparison, ELISA is more suitable for serum HBsAg detection in hospitals and blood centres.

关 键 词：胶体金免疫层析法 酶联免疫吸附试验 乙型肝炎病毒表面抗原 

分 类 号：R446.1[医药卫生—诊断学] R512.62[医药卫生—临床医学]