银杏枝条营养贮藏蛋白质的组分及动态变化  

Components and Dynamics of Vegetative Storage Proteins in the Branch of Ginkgo biloba

作　　者：郭红彦[1,2] 吴青霞[2] 彭方仁[2] 

机构地区：[1]山西农业大学林学院,太谷030801 [2]南京林业大学森林资源与环境学院,南京210037

出　　处：《林业科学》2009年第3期24-28,171,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30872055);高校博士点基金(20070298008);山西农业大学科技创新基金项目(2008029)

摘　　要：采用SDS-聚丙烯酰胺凝胶电泳技术和电子显微镜技术,对银杏枝条营养贮藏蛋白质组分及其动态变化规律进行研究。结果表明:银杏营养贮藏蛋白质主要存在于枝条的树皮和木质部中;当年生枝条含量高于2年生枝条,树皮的含量高于木质部;枝条中营养贮藏蛋白质组分主要是分子质量为36ku和32ku的蛋白质;银杏营养贮藏蛋白质的动态变化分为积累和降解2个阶段,5月中旬新叶形成后即开始积累,12月份达到最高,翌年春季降解满足芽的萌发,至4月上旬逐渐消失。The components and dynamics of vegetative storage proteins（VSPs） in branches of Ginkgo biloba were investigated by SDS-PAGE and electronic microscopy. The results showed that VSPs localized mainly in bark and xylem of branches. The content of VSPs in one-year-old branches was higher than that in two-year-old branches, and the content of VSPs in bark was higher than that in xylem. The components of VSPs in branches were composed of molecular weight 36 ku and 32 ku proteins. The seasonal fluctuation of VSPs could be divided into accumulation and degradation two stages. In the accumulation stage, the VSPs were accumulated in the middle of May with generation of the new leaves, and its content reached the peak in December. In the degradation stage, the VSPs were degraded to meet the need of buds-sprouting in the next spring, and degraded completely in the beginning of April.

关 键 词：银杏 营养贮藏蛋白质 季节变化 SDS-聚丙烯酰胺凝胶电泳 电子显微镜 

分 类 号：S718.43[农业科学—林学]