毛竹捕光叶绿素a/b结合蛋白基因cab-PhE1的克隆与表达分析  

Cloning and High Prokaryotic Expression of the Light Harvesting Chlorophyll a/b-Binding Protein Gene from Bamboo(Phyllostachys edulis)

作　　者：高志民[1] 刘成[2] 刘颖丽[1] 彭镇华[3] 

机构地区：[1]国际竹藤网络中心国家林业局竹藤科学与技术重点开放实验室,北京100102 [2]中国科学院植物研究所,北京100093 [3]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学》2009年第3期145-149,共5页Scientia Silvae Sinicae

基　　金：国家林业局948项目(2004-4-58);国家林业局重点项目(2007-1)

摘　　要：The light harvesting chlorophyll a/b-binding protein is one of key proteins in the transformation from light energy to chemical energy. An open reading frame coding precursor protein of cab gene was cloned from the first strand of bamboo cDNA through RT-PCR methods,and named as cab-PhE1 (cab gene 1 from Phyllostachys edulis EF207229). The sequence analysis showed that the deduced polypeptide was highly homologous to some other CAB proteins from monocotyledon,and the gene belonged to lhcb2 family. Tissue specific expression showed that cab-PhE1 expressed higher in leaf than sheath and stem. The prokaryotic expression vector of cab-PhE1 gene encoding the mature protein was constructed by subcloning the fragment into pET-23 a and was expressed in Escherichia coli induced by IPTG. The molecular weight of the induced protein was about 28 ku,approximate to that of the mature protein. This work is a key to the further research on in vitro reconstitution of light-harvesting Chl a/b complexes.The light harvesting chlorophyll a/b-binding protein is one of key proteins in the transformation from light energy to chemical energy. An open reading frame coding precursor protein of cab gene was cloned from the first strand of bamboo eDNA through RT-PCR methods, and named as cab-PhE1 （cab gene 1 from Phyllostachys edulis EF207229） . The sequence analysis showed that the deduced polypeptide was highly homologous to some other CAB proteins from monocotyledon, and the gene belonged to lhcb2 family. Tissue specific expression showed that cab-PhE1 expressed higher in leaf than sheath and stem. The prokaryotic expression vector of cab-PhE1 gene encoding the mature protein was constructed by subcloning the fragment into pET- 23 a and was expressed in Escherichia coli induced by IPTG. The molecular weight of the induced protein was about 28 ku, approximate to that of the mature protein. This work is a key to the further research on in vitro reconstitution of light-harvesting Chl a/b complexes.

关 键 词：毛竹 捕光叶绿素a/b结合蛋白基因 组织特异性表达 原核表达 

分 类 号：S718.46[农业科学—林学] Q943.2[生物学—植物学]