液相色谱─间接光度检测测定脂族醇  

Determination of aliphatic alcohols by liquid chromatography and indirect photometric detection

作　　者：张孝松[1] 徐霁昀[1] 方冶 郭玉蓉 林长山[1] 

机构地区：[1]中国科学技术大学化学系

出　　处：《化学试剂》1998年第1期31-33,共3页Chemical Reagents

摘　　要：在化学键合固定相ＯＤＳ柱（２５０ｍｍ×４．６ｍｍ内径）上，用Ｒｕ（Ｐｈｅｎ）２＋３作甲醇┐水（８９２，Ｖ／Ｖ）流动相添加剂分离并用间接光度法检测某些脂族醇。探讨了流动相中有机溶剂浓度、Ｒｕ（Ｐｈｅｎ）２＋３浓度、ｐＨ值和盐浓度等对醇类的保留时间和检测灵敏度的影响。甲醇、乙醇、正丙醇、异丙醇、正丁醇和叔丁醇具有良好的分离度和检测灵敏度，间接光度法检测可在４５０ｎｍ处，也可在３３０ｎｍ处。同时测定此６种醇的校正曲线线性范围，甲醇、乙醇各为０．５～５０ｍｇ／ｍＬ，其余４种醇各为１～１００ｍｇ／ｍＬ，绝对检测限达微克级。On a Zorbax ODS column (250 mm×4 6 mm I.D.),Ru(Phen) 2+ 3 was used as a mobile phase additive for the liquid chromatographic separation of low molecular weight aliphatic alcohols.The effect of organic modifier,concentration of Ru(Phen) 2+ 3 and salt,and pH value of the mobile phase on the retention time and detection sensitivities of the analyte alcohols have been investigated.Good resolutions of methanol,ethanol,1 propanol,2 propanol, n butanol and tert butyl alcohol were obtained by using methanol water (8∶92, V/V ) containing 1 0×10 -4 mol/L Ru(Phen) 2+ 3 as the mobile phase at a flow rate of 1 0mL/min.Indirect photometric detection was used to detect the analyte alcohols by monitoring the eluant at 450 nm,or at 330 nm.The calibration graphs were linear over 0 5～50 mg/mL for methanol and ethanol,and 1～100 mg/mL for 1 propanol,2 propanol, n butanol and tert butyl alcohol.The detection limits were of the order of microgram for the alcohols.

关 键 词：液相色谱 间接光度检测 脂肪醇 脂肪族醇 

分 类 号：O623.41[理学—有机化学]