流动注射化学发光法测定利血平  

Determination of Reserpine by Flow-injection Chemiluminescence Analysis

作　　者：李丽清[1] 杨敏丽[2] 封满良[3] 吕九如[3] 

机构地区：[1]山东泰安师范专科学校化学系,271000 [2]宁夏大学化学系,750021 [3]陕西师范大学化学系,西安710062

出　　处：《分析化学》1998年第3期307-309,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目

摘　　要：研究了利血平在酸性条件下与高锰酸钾和过氧化氢产生化学发光的行为，建立了流动注射化学发光测定利血平的新方法。利血平的浓度在１．０×１０－６～８．０×１０－５ｇ／ｍＬ范围内与化学发光强度呈良好的线性关系；检出限为３×１０－７ｍ／ｍＬ。对６×１０－６ｇ／ｍＬ利血平进行１１次平行测定，得方法的相对标准偏差为１．３％。方法用于药剂中利血平含量测定，结果与药典标准方法测得值一致。An analytical method consisting of flow-injection sampling and chemiluminescence detection for determination of reserpine is described. It is based on the chemiluminescence reaction of KMnO4-H2O2-reserpine system. The chemiluminescence intensity was correlated linearly with concentration of reserpine in the range of 1.0×10-6 to 8.0×10-5 g/mL. The relative standard deviation is 1.3% for 11 measurements of 6.0×10-6g/mL reserpine standard solution. The method has been applied to the determination of reserpine in pharmaceutical preparations and the results are in good agreement with that by standard method.

关 键 词：利血平 流动注射 化学发光 

分 类 号：R971.4[医药卫生—药品] R972.4[医药卫生—药学]