姜黄素对中波紫外线损伤HaCaT细胞保护作用  

Protective effect of curcumin on damage of HaCaT cells irradiated with UVB

作　　者：康顺爱[1] 郭彩霞[1] 王志成[1] 李艳博[1] 姚崇舜[2] 龚守良[1] 李校堃[2] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]温州医学院药学院

出　　处：《中国公共卫生》2009年第4期422-423,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30570546)

摘　　要：目的通过姜黄素(Cur)影响中波紫外线(UVB)辐射HaCaT细胞线粒体膜电位(Δψ)及其相关因子,探讨其对UVB损伤细胞的保护作用及其机制。方法HaCaT细胞经UVB辐射后,加入不同剂量姜黄素继续培养,通过罗丹明123和Fluo-3/AM荧光强度检测细胞Δψ电位和细胞内游离钙离子浓度([Ca2+]i),以流式细胞术检测caspase-3和细胞色素c(Cytc)凋亡相关蛋白的表达。结果UVB辐射HaCaT细胞后,低线粒体膜电位的细胞数为(80.8±2.23)%,[Ca2+]i为(71.9±1.61)%,caspase-3和Cytc蛋白表达量分别为(29.00±1.60)%和(27.5±1.60)%,与对照组比较,差异均有统计学意义(P<0.01);加入姜黄素后与UVB组比较,细胞线粒体膜电位、[Ca2+]i及Cytc和caspase-3蛋白表达均呈剂量-效应关系,即随着药物浓度的增加,具有低细胞线粒体膜电位的细胞数、Cytc和caspase-3蛋白表达均显著减少,[Ca2+]i显著降低。结论姜黄素可能通过提高细胞线粒体膜电位,降低[Ca2+]i含量,减弱Cytc和caspase-3的级联反应,达到其抑制UVB辐射引起的HaCaT细胞凋亡,具有对UVB损伤HaCaT细胞的保护作用。Objective To study the effect of curcumin （Cur） on the mitochondria and some correlation factors in HaCaT cells irradiated with UVB and its protection effect on the cells damaged by UVB. Methods After different doses of Cur were added into HaCaT cells irradiated with UVB, the cells were continually cultured. The mitochondria membrane potential （△ψ） and the free intracellular calcium （ [ Ca^2＋]i） concentration were measured with Rhodamine 123 and Fluo -3/ AM fluorescence,respectively. The protein expressions of caspase-3 and cytochrom c （Cyt c） were examined by flow cytometry. Results As compared with those in the normal control group after UVB irradiation, all indices increased significandy（ P 〈 0.01 ）. The cell population of olig-△ψ potential was 80. 8 ± 2.23% ; the [Ca^2＋] i concentration was 71.9 ± 1.61% and the protein expressions of caspase-3 and Cyt c were 29. 00 ± 1.60% and 27. 5 ± 1.60% ,respectively. As compared with those in the UVB group, a down-regulation dose-effects of Cur in the △ψ potential and [ Ca^2＋ ] i concentration and the protein expressions of caspase-3 and Cyt c were shown. Conclusion The results suggest that Cur could inhibit HaCaT cell apoptosis caused by UVB irradiation through the increase of △ψ potential and the decreases of the [ Ca^2＋ ] i concentration and the cascade reaction of Cyt c and caspase-3, and have protective effect on HaCaT cells damaged by UVB.

关 键 词：姜黄素 HACAT细胞 中波紫外线 线粒体 膜电位 

分 类 号：R122.25[医药卫生—环境卫生学]