姜黄素对肝星状细胞增殖能力影响  

Effects of curcumin on proliferation of hepatic satellite cell in vitro

作　　者：纪辉[1] 任立群[2] 张一宁[3] 李相军[2] 石艳[3] 迟宝荣[1] 

机构地区：[1]吉林大学第一医院消化内科,吉林长春130021 [2]吉林大学药学院实验药理与毒理实验室 [3]吉林大学第一医院儿内一科

出　　处：《中国公共卫生》2009年第4期426-427,共2页Chinese Journal of Public Health

基　　金：吉林省科技厅基金项目(20030436)

摘　　要：目的探讨姜黄素对体外培养大鼠肝星状细胞细胞株(CSFSC-2G)增殖能力及细胞外基质分泌的影响。方法四甲基偶氮噻唑蓝(MTT)法检测不同浓度姜黄素(5,10,15,20,40μmol/L)在不同培养时间作用下肝星状细胞(HSC)的增殖能力;酶联免疫吸附实验法检测不同浓度姜黄素作用下HSCI型胶原(CollagenI,ColI)和III型胶原(CollagenIII,ColIII)合成及分泌能力;流式细胞术检测姜黄素对HSC凋亡的影响。结果不同浓度的姜黄素(5,10,15,20,40μmol/L)均可抑制HSC增殖,并呈现明显剂量依赖关系;含20μmol/L姜黄素培养基培养24h后,HSC-ColI、ColIII吸光度(A)值分别为(0.418±0.019),(0.554±0.043),均明显低于阴性对照组,差异有统计学意义(P<0.05);含20μmol/L姜黄素培养基培养48h后,HSC处于G0/G1期的HSC细胞占(39.98±8.07)%,S期细胞占(55.79±9.13)%,G2/M期细胞占(4.23±0.12)%;凋亡率为(20.31±2.55)%,高于阴性对照组凋亡率(0.22±0.11)%,低于秋水仙素阳性药物对照组凋亡率(29.75±2.64)%,差异均有统计学意义(P<0.05)。结论姜黄素可抑制HSC细胞的增殖并促进其凋亡,有可能成为防治肝纤维化的理想药物。Objective To investigate the effects of curcumin on proliferation and collagen （col） secretion of hepatic satellite cells in vitro. Methods CSFSC - 2G were incubated with different concentration of curcumin （ 0,5,10,15,20,40 μmol/L） for different times （6h,12h,24h,48h and 72h）. MTT was used for the detecting of proliferation of HSC. Col Ⅰ and Col Ⅲ were determined by ELISA and flowcytometry was applied to evaluate apoptosis of HSC. Results Curcumin inhibited the proliferation of HSC with dose-dependent manner, especially in 20 and 40 μmoL/L curcumin groups at 24h. When HSC was cultured after 24h, the absorbency of Col Ⅰ and Col Ⅲ in 20 μmol/L curcumin group was 0. 418 ± 0. 019 and 0. 554 ±0. 043 ,respectirely,both lower than those of 0 μmol/L curcumin group significantly （ P 〈 0. 01 ）. When HSC was cultured after 48h,the ratio of apoptosis in 20 μmol/L curcumin group was 20. 31% ± 2.55% and higer than that of 0 μmol/L curcumin group significantly （ P 〈 0. 01 ）. Conclusion Curcumin can delay liver fibrosis not only by inhibiting the proliferation and stimulating apoptosis of HSC, but also by decreasing Col Ⅰ and Col Ⅲ secretion.

关 键 词：姜黄素 肝星状细胞 肝纤维化 

分 类 号：R996.2[医药卫生—毒理学] R281.5[医药卫生—药学]