疏水腔修饰法及高活力GPX抗体酶的研究──Ⅱ.半抗原及全抗原的制备和表征  

Hydrophobic Pocket Modification and Glutathione Peroxidase Abzyme with High Activity-Ⅱ.Synthesis and Characterization of Haptens and Antigens

作　　者：马雪艳[1] 王琳[1] 丁兰[1] 赵大庆[1] 倪嘉缵[1] 

机构地区：[1]中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春130022

出　　处：《应用化学》1998年第1期53-55,共3页Chinese Journal of Applied Chemistry

基　　金：中国科学院基础局重大资助;吉林省科委重点资助

摘　　要：The thiol group of glutathione (GSH) was protected by 2, 4-dinitrochlorobenzene(DNCB). The product GSH-S-DNP was reacted with 2-methyl-1-propanol and 3-methyl-1-butanol respectively to obtain hapten6 and 7. Their structures were determined by 400 MHz1H NMR and IR. The two haptens were then covalently crosslinked with bovine serum albu-min(BSA) hy using glutaraldehyde as coupling agent to obtain antigen 6 and 7. The resultsof gel electrophoresis analysis gave the average molecular weight of 1. 27 ×10-22 kg for eachantigen. The average connected ratio of BSA to hapten was 1: 12. Other UV and fluores-cence spectral properties of the antigens are reported.The thiol group of glutathione (GSH) was protected by 2, 4-dinitrochlorobenzene(DNCB). The product GSH-S-DNP was reacted with 2-methyl-1-propanol and 3-methyl-1-butanol respectively to obtain hapten6 and 7. Their structures were determined by 400 MHz1H NMR and IR. The two haptens were then covalently crosslinked with bovine serum albu-min(BSA) hy using glutaraldehyde as coupling agent to obtain antigen 6 and 7. The resultsof gel electrophoresis analysis gave the average molecular weight of 1. 27 ×10-22 kg for eachantigen. The average connected ratio of BSA to hapten was 1: 12. Other UV and fluores-cence spectral properties of the antigens are reported.

关 键 词：抗体酶 半抗原 制备 全抗原 疏水腔修饰 GSH GPX 

分 类 号：Q553.03[生物学—生物化学]