血必净注射液联合前列腺素E_1防治急性肺纤维化的实验研究  

Experiment Research of Xuebijing Injection and Prostaglandin E_1 in Prevention and Cure Acute Pulmonary Interstitial Fibrosis

作　　者：刘红栓[1] 李志军[2] 

机构地区：[1]北京市通州区中医医院,北京101100 [2]天津市第一中心医院,天津300192

出　　处：《中国中医急症》2009年第4期584-586,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：天津市科委资助项目(No.043608811)

摘　　要：目的探讨血必净注射液联合前列腺素E1对实验大鼠急性肺间质纤维化的防治作用及其机制。方法采用一次性气管内注射博莱霉素的方法复制大鼠肺间质纤维化模型。将大鼠随机分为四组,分别予以相应治疗,各组动物处死后分离肺组织称重,计算湿肺系数,测定羟脯氨酸含量,行支气管肺泡灌洗并收集灌洗液用ELISA方法测定肿瘤坏死因子α(TNF-α)水平。结果血必净注射液联合前列腺素E1能减少羟脯氨酸含量,抑制TNF-α表达的异常升高,减轻肺泡炎和肺纤维化程度。结论血必净注射液联合前列腺素E1能有效改善实验性肺纤维化大鼠的病理改变,并延缓其恶化进程。其机制可能是通过抑制TNF-α的表达,减轻早期炎症反应并减少胶原蛋白的产生,使炎症及纤维化病变减轻。Objective： To observe the therapeutic effect and mechanism of Xuebijing lnjection（XBJI） and Prostaglandin E1 （PGE1） in prevention and cure acute pulmonary interstitial fibrosis（PIF） rats. Methods： Acute PIF model rats were established by intratracheal injection of bleomycin. The model rats were randomly divided into four groups. The groups were given separately corresponding treatment. The pathological changes, levels of hydroxypro- line（HYP） were measured. Bronchoalveolar lavage were collects and ELISA was applied for tumor necrosis factor -α quantitation. Results： XBJI and PGE1 could inhibit abnormal heighten of HYP content and TNF - α expression. Amelioration of the pulmonary alveolitis and fibrosis were shown after treatment by XBJI and PGE~. Conclusion： XBJI and PGEt have definite effects in treating acute PIF. The mechanism may be through inhibiting the TNF -α expression, decreasing collagen protein, so as to ameliorate the inflammation and fibrosis.

关 键 词：血必净注射液 前列腺素E1 肺纤维化 羟脯氨酸 肿瘤坏死因子Α 大鼠 

分 类 号：R285.5[医药卫生—中药学]