长白山木贼山柰素含量对反射光谱参数的响应  

Responses of kaempferol content in Hippochaete hiemale from Changbai Mountains to spectral reflectance parameters.

作　　者：朱小迪[1] 范秀华[1] 李永慈[1] 

机构地区：[1]北京林业大学,北京100083

出　　处：《生态学杂志》2009年第4期607-612,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(30771716);国家“十一五”科技支撑资助项目(2006BAD03A0804)

摘　　要：对长白山地区木贼山柰素含量和反射光谱参数的关系进行了研究,探索了判别木贼药材质量优劣的快捷方法。根据反射光谱曲线(Unispec-Sc光谱分析仪)导出的若干参数,对木贼样品进行聚类分析,采用HPLC法分别测定木贼山柰素的含量,分析了反射光谱参数与山柰素含量的相关性。结果表明:蓝边面积(SDb)、绿峰反射率(Rg)等参数与山柰素含量呈显著负相关(r分别为-0.991,-0.982),530～570nm波段的反射率与山柰素含量亦呈显著的负相关(︱r︱>0.95)。通过比较SDb、Rg,可简捷地判断长白山地区木贼质量的优劣。增强530～570nm波段的入射光线,可刺激山柰素含量的增加,但需要大田实验进一步验证。This paper studied the relationships between kaempferol content in Hippochaete hiemale from Changbai Mountains and spectral reflectance parameters, aimed to establish a shortcut method for judging the medicinal quality of this plant. According to the spectral reflectance parameters derived from the reflectance spectral curves determined by Unispec-Sc spectral analysis system, cluster analysis was conducted for H. hiemale samples. The correlation analysis between the kaempferol content of this plant determined by HPLC and the spectral reflectance parameters showed that the kaempferol content had significant negative correlations with the area of blue edge （SDb） （r = -0. 991 ） and the reflectance of green peak （Rg） （r = -0. 982 ）, as well as with the reflectance of 530-570 nm （ ｜ r ｜ 〉0.95）. As a result, the medicinal quality of H. hiemale from Changbai Mountains could be judged rapidly by comparing the SDb and Rg. Furthermore, to strengthen the incident light of 530-570 nm could stimulate the increase of kaempferol content in Hippoehaete hiemale, but it needs to be confirmed by field test.

关 键 词：木贼 山柰素含量 光谱反射 相关性 

分 类 号：S567.239[农业科学—中草药栽培]