拮抗放线菌F-15的生防作用及其初步分类鉴定  

Biocontrol Effect and the Taxonomy of Antagonistic Actinomyces Strain F-15

作　　者：朱春玉[1] 李辉[1] 李立梅[2] 邹志远[1] 孟庆杰[1] 刘宏生[1] 

机构地区：[1]辽宁大学生命科学院动物资源与疫病防治重点实验室,沈阳110036 [2]沈阳农业大学植物保护学院,沈阳110161

出　　处：《农药》2009年第4期301-303,共3页Agrochemicals

基　　金：国家科技部863计划项目子专题(编号:2001AA247011)

摘　　要：从沈阳东陵区蔬菜大棚土壤中分离到1株拮抗放线菌,编号为F-15。采用平板对峙培养法和温室盆栽防治法测定了其抑菌活性,并通过形态学、化学的方法初步研究了其分类地位。结果表明:该菌株对供试的多种植物病原菌均有抑制作用,其中对瓜类枯萎病菌和番茄叶霉病菌抑制作用较强,平均抑菌带宽度达到14.8￣15.0mm。温室防病实验结果表明:其发酵上清液对番茄叶霉病具有良好的生防作用,防治效果可以达到73.2%。根据其菌株形态、培养特征、生理生化特性、细胞壁化学组分等分析,将菌株F-15归入链霉菌属,并初步鉴定为不吸水链霉菌的金色类群Streptomyces aureus。An antagonistic actinomyces strain F-15 was isolated from vegetable soil in suburb in Shengyang. The fungistatic activity of F-15 was determined by confronting incubation on PDA plates and control test to the potted plants in greenhouse, and the taxonomic identification of the strain was carried out with morphological and chemical methods. The results showed that the strain F-15 displayed a broad-spectrum inhibition activity to all the 9 plant pathogenic fungi tested. Especially, the inhibition zones out to the width of 14.8-15.0 mm were presented by the strain against 2 ones of the pathogens, Fusarium oxysporum and Fulviafulva The biocontrol effect of strain F-15 to leaf mold of tomato reached 73.2% in greenhouse. According to the characteristics of morphological, cultural, physiological and biochemical, chemical components of cell wall, the strain F-15 was identified as Streptomyces aureus.

关 键 词：链霉菌 分类鉴定 生防作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治]