链霉素人工抗原的制备与鉴定  

Preparation and Idenfication of Artificial Antigen of Streptomycin

作　　者：范国英[1] 王顺岗[1] 王自良[1] 邓润广[2] 张改平[2] 杨艳艳[2] 

机构地区：[1]河南科技学院动物科学学院,河南颏乡453003 [2]河南省农业科学院免疫学重点实验室,河南郑州450002

出　　处：《西北农业学报》2009年第2期45-50,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：“十一五”国家重大项目:畜禽及其制品安全生产的质量控制技术研究(2006BAK02A21)

摘　　要：链霉素经氧-羧甲基羟胺肟化处理后引入羧基,采用EDC一步法分别合成免疫原BSA-EDC-SM和包被抗原OVA-EDC-SM;用戊二醛法和1,4-丁二醇缩水甘油醚分别合成BSA-GA-SM和BSA-di-SM;采用SDS-PAGE凝胶电泳和紫外扫描测得BSA-EDC-SM、BSA-GA-SM和BSA-di-SM的BSA与SM的分子结合比分别为1∶23、1∶15和1∶10。用3种免疫原免疫Balb/C小鼠,获得了高价、敏感和特异的抗血清。BSA-EDC-SM免疫组中1#小鼠的多克隆抗体对SM的IC50为45.8μg/mL,对双氢链霉素(DHSM)的交叉反应为90.6%,与其他氨基糖苷类药物或抗生素药物的交叉反应均小于0.001%。BSA-di-SM和BSA-GA-SM免疫组的多克隆抗体对DHSM、其他氨基糖苷类药物或抗生素类药物的交叉反应均小于0.001%。Immunogen BSA-EDC-SM and coating antigen OVA-EDC-SM were synthesised by using EDC one-step after inleted carboxy group by O-carboxymethyl-hydroxylamine,BSA-GA-SM and BSA-di-SM were synthesized by using glutaraldehyde and 1,4-butanediol diglycidyl ether.Corresponding identification was made by SDS-PAGE and UltraViolet scan.Bind score molecule of BSA and SM of BSA-EDC-SM,BSA-GA-SM,BSA-di-SM were 1∶23,1∶15,1∶10 respectively.The high-titer,sensitive and specific SM antiserum had been produced by immunized 6W Balb/C mice.IC50 of pAb of BSA-EDC-SM immunity class miceⅠ-1# anti-SM was 45.8 μg/mL,cross reaction rate to DHSM was 90.6%,and no cross reaction to other antibiotics.BSA-di-SM、BSA-GA-SM immunity class mice were no cross reaction to DHSM and other antibiotics.

关 键 词：链霉素 人工抗原 制备 鉴定 

分 类 号：S859.84[农业科学—临床兽医学]