油菜ATP硫酸化酶部分cDNA克隆及序列分析  

Cloning and Sequence Analysis of Partial cDNA of ATPS from Brassica napus

作　　者：王帮庆[1,2] 邱睿[1] 王保莉[1] 

机构地区：[1]西北农林科技大学生命科学院,陕西杨凌712100 [2]汉中植物研究所,陕西汉中723000

出　　处：《西北农业学报》2009年第2期126-130,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：西北农林科技大学创新团队项目资助

摘　　要：ATP硫酸化酶(ATP sulfurylase,ATPS,EC 2.7.7.4)广泛存在于动物、植物、微生物中,可催化ATP和SO42-生成APS和PPi,也可以催化此过程的逆反应。用Genbank公布的的油菜ATPS全长序列与甘蓝、拟南芥以及洋葱的序列进行比对,得到一段保守性比较强的序列。采用RT-PCR方法从油菜‘秦优8号’的叶片获得880 bp长的cDNA片段,命名为RATPS_8。序列分析和比对结果表明:RATPS_8与已报道的甘蓝、芥菜和油菜的基因同源性分别为95%、92%和91%,进化树聚类分析和预测氨基酸的BLAST结果证实,RATPS_8为油菜‘秦优8号’ATP硫酸化酶的部分基因片段。ATP sulfurylase（ATPS,EC 2.7.7.4）,which exists widely in animals,plants and microorganisms,can catalyze ATP and SO42-into APS and PPi,while reversing this reaction.After alignment of the ATPS gene sequence of Brassica napus with of those of rape,cabbage,Arabidopsis thaliana and onion submitted on Genbank,a highly conserved sequence was selected as target fragment for cloning.The fragment was amplified by RT-PCR from cDNAs of leaves of rape ＇Qinyou8＇,named as RATPS_8.Sequencing analysis showed that RATPS_8 shared identity of 95%,92% and 91% with ATPS gene of Brassica oleracea,Brassica juncea and Brassica napus,respectively.Analysis of phylogenetic tree and alignment of predicted amino sequence indicated that RATPS_8 is the partial ATP sulfurylase gene of rape ＇Qinyou8＇.

关 键 词：油菜 ATP硫酸化酶 CDNA 基因克隆 序列分析 

分 类 号：S565.4[农业科学—作物学]