高效液相色谱法测定制剂中本芴醇含量  

Determination of benflumetol in its preparations by HPLC with UV detection

作　　者：吴婧[1] 王向[1] 白克华[1] 张淑秋[1] 

机构地区：[1]山西医科大学药学院临床药学教研室,太原030001

出　　处：《山西医科大学学报》2009年第3期250-253,共4页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(30572367)

摘　　要：目的建立本芴醇的含量测定方法。方法采用反相高效液相-紫外检测法,流动相为甲醇∶水∶冰醋酸∶二乙胺(体积比94.5∶4∶1∶0.5),流速0.8ml/min,检测波长335nm。结果本芴醇在2.0-200μg/ml范围内线性关系良好,以峰面积对本芴醇浓度进行线性回归,方程为A=43088C+49741,r=0.9998;日内、日间精密度RSD均小于2%;平均回收率在98%-102%之间。结论该高效液相色谱法实验操作简单方便,准确度、精密度及重现性等均符合药典要求,可为各种制剂中本芴醇的质量控制提供依据。Objective To establish a method for determining the content of benflumetol in its preparations by HPLC with UV detection. Methods HPLC method was used with methanol-water-glacial acetic acid-diethylamine（94.5∶4∶1∶0.5,V/V/V/V）as the mobile phase at a flow rate of 0.8 ml/min.UV detection wave length was set at 335 nm. Results Benflumetol showed a good linearity in the range of 2.0-200 μg/ml.The lower limit of quantification was 2.0 μg/ml.The intra-day and inter-day RSDs were less than 2%.Mean recovery was 98.0%-102.0%. Conclusion The method is simple,accurate and precise,and could meet the requirement of quality control of benflumetol in its preparation.

关 键 词：本芴醇 高效液相色谱法 含量测定 

分 类 号：R927.1[医药卫生—药学]