光合细菌转化复方决明子口服液中蒽醌类成分的含量测定  

Determination of the content of anthraquinones in compound cassia seed oral solution transformed by photosynthetic bacteria

作　　者：秦娟[1] 李利华[2] 杨官娥[1] 张肇铭[3] 赵丽华 吉海 

机构地区：[1]山西医科大学药学院中药学教研室,太原030001 [2]山西医科大学药学院 [3]山西大学生命科学与技术学院 [4]山西维民生科技有限公司

出　　处：《山西医科大学学报》2009年第3期254-256,共3页Journal of Shanxi Medical University

基　　金：山西省科技攻关基金资助项目(051081);山西省教育厅高科技开发基金资助项目(20051227);2007年山西医科大学创新基金资助项目;2008年山西省教育厅大学生创新性实验基金资助项目

摘　　要：目的建立光合细菌转化复方决明子口服液中游离蒽醌和结合蒽醌的含量测定方法。方法用醋酸镁甲醇显色,以大黄酚为对照品,采用紫外可见分光光度法在所选最大吸收波长510nm处测定光合细菌转化复方决明子口服液中蒽醌类成分的含量。结果在2-12μg/ml范围内线性关系良好,回归方程为A=0.0579C+0.0424,r=0.9997;游离蒽醌平均回收率97.8%,RSD=2.4%。结论该紫外可见分光光度法简便、准确,可用于光合细菌转化复方决明子口服液中蒽醌类成分的含量测定。Objective To establish a content determination method of free anthraquinone and conjugated anthraquinone in compound cassia seed oral solution transformed by photosynthetic bacteria. Methods With magnesium acetate as color developing reagent and chrysophanol as reference substance,the content of anthraquinones in compound cassia seed oral solution transformed by photosynthetic bacteria was determined at the maximum absorption wavelength of 510 nm by UV-Vis spectrophotometry. Results The linear correlation was good in the range of 2-12 μg/ml,and the regression equation was A=0.057 9C＋0.042 4（r=0.999 7）.The average reco-very of free anthraquinone was 97.8% with RSD 2.4%（n=6）. Conclusion The UV-Vis spectrophotometry is simple,accurate and suitable for determining the content of anthraquinones in compound cassia seed oral solution transformed by photosynthetic bacteria.

关 键 词：光合细菌 决明子 蒽醌 含量测定 

分 类 号：R927.2[医药卫生—药学]