复合螺旋藻多糖对人宫颈癌HeLa细胞的抑制作用  

Inhibitory Effects of Composite PSP on HeLa Cells of Human Cervical Cancer

作　　者：郝言芝[1] 张志娟[2] 乔明霞[3] 高宗霞 于蕾妍[2] 王清吉[2] 

机构地区：[1]青岛农业大学校医院,山东青岛266109 [2]青岛农业大学生命科学学院,山东青岛266109 [3]山东省肿瘤医院放射科,山东青岛250117 [4]山东省平度市长乐镇动检所,山东平度266714

出　　处：《安徽农业科学》2009年第8期3367-3368,3408,共3页Journal of Anhui Agricultural Sciences

基　　金：青岛市科技发展计划(第2批)项目(06-2-2-12-jch);青岛农业大学高层次人才启动基金(630432)资助

摘　　要：[目的]探讨复合螺旋藻多糖对人宫颈癌HeLa细胞增殖的影响。[方法]将螺旋藻多糖(PSP)与银杏叶有效成分(GBE)按不同剂量、3种比例(1∶1、1∶2和2∶1)复合,用MTT法研究复合螺旋藻多糖对人宫颈癌HeLa细胞的抑制作用。[结果]PSP与GBE按3种比例复合使用组对人宫颈癌HeLa细胞的抑制作用均明显好于相对应剂量的单一成分组,且同一配比组的抑瘤率与剂量呈正相关。PSP与GBE联合使用能对抑制人宫颈癌HeLa细胞产生协同增效作用。其中PSP与GBE按2∶1比例复合的作用最突出,在高、中和低剂量时抑瘤率分别为67.92%、58.93%和34.86%(P<0.01)。[结论]该研究为人宫颈癌的治疗奠定了基础。[ Objective ] The aim was to discuss the effect of composite PSP on the proliferation of HeLa cells of human cervical cancer. [ Method ] PSP and GBE were compounded according to different doses and 3 proportions of 1 ： 1, 1：2 and 2：1, and the inhibitory effect of the composite PSP on HeLa cells of human cervical cancer was studied by MTT method. [ Result ] The inhibitory effects of using PSP and GBE compoundly according to 3 proportions on HeLa cells of human cervical cancer were all obviously better than that of using single component at corresponding doses and the tumor inhibition rate of group at the same proportion showed positive correlation with dose. The combined use of PSP and GBE could produce synergy in inhibiting HeLa ceils of human cervical cancer. Among them, the effect of PSP and GBE with the pro- portion at 2：1 was most prominent and the tumor inhibition rates at high, middle and low doses were 67.92%, 58.93% and 34.86% （ P 〈 0. 01 ） , resp. [ Conclusion] The study laid a foundation for curing human cervical cancer.

关 键 词：螺旋藻多糖 银杏 复配 抑制作用 

分 类 号：Q949.22[生物学—植物学]