新疆红花组织培养与快速繁殖的研究  

Study on the Tissue Culture and Rapid Propagation of Carthamus tinctorius

作　　者：杨晶[1] 邵明龙[1] 李天航[1] 李校堃[1] 

机构地区：[1]吉林农业大学教育部生物反应器与工程研究中心,吉林长春130118

出　　处：《安徽农业科学》2009年第8期3439-3440,共2页Journal of Anhui Agricultural Sciences

基　　金：国家科技部"863"计划"植物生物反应器专项"

摘　　要：[目的]筛选新疆红花组织培养的调控体系,以获得离体培养的再生植株。[方法]将无菌材料置于不同的培养基上培养构建愈伤组织无性系,进行继代培养,最终将生根的炼苗移栽至基质中使其生长。[结果]子叶是用于红花再生较理想的材料,愈伤组织的分化能力在不同培养基上明显不同。筛选出的适合红花建立再生体系的最佳诱导培养基为MS+1.0 mg/L NAA+2.0 mg/L BA+3.0%蔗糖+0.7%琼脂,分化培养基为MS+0.2 mg/L NAA+2.0 mg/L BA+3.0%蔗糖+0.7%琼脂,生根培养基为1/2 MS+2mg/L IBA+0.2 mg/L BA。[结论]该研究为红花的资源保护、扩大繁殖和进一步推广应用及利用其作受体系统研究植物生物反应器奠定了基础。[ Objective ] The aim was to screen out regulation system for the tissue culture of Carthamus tinctorius so as to obtain regenerated plant in vitro culture. [ Method ] The asepsis materials were cultured in different media to construct the callus clones, and then subcuhured and finally the rooting hardening seedlings were transplanted in substance for their growing. [ Resuh] Cotyledon was the relatively ideal material for the regeneration of C. tinctorius. The differentiation abilities of calli were different obviously in different media. The screened optimum media suitable for C. tinctorius to establish its regeneration system were as follows： induction medium MS ＋ 1. 0 mg/L NAA ＋ 2.0 mg/L BA ＋ 3.0% sucrose ＋ 0.7% agar, differentiation medium MS ＋ 0.2 mg/L NAA ＋ 2.0 mg/L BA ＋ 3.0% sucrose ＋ 0. 7% agar and rooting medium 1/2 MS ＋ 2.0 mg/L IBA ＋ 0.2 mg/L BA. [ Conclusion] The research laid a foundation for the resource protection, enlarged propagation, further popularization and application and for researching the plant bioreactor with it as the receptor system.

关 键 词：红花 组织培养 培养基 

分 类 号：S603.6[农业科学—园艺学]