野生花卉厚叶岩白菜组织培养及再生体系的建立  

Tissue Culture of Wild Flower Bergenia crassifolia and Establishment of Its Regeneration System

作　　者：刘敏[1] 郝秀英[1] 徐琴[1] 波拉提[1] 康喜亮[1] 王晓军[1] 

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011

出　　处：《安徽农业科学》2009年第8期3455-3456,3462,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立厚叶岩白菜的完整再生体系,并为研究其遗传转化奠定基础。[方法]以野生厚叶岩白菜的叶片为外植体,通过筛选培养基与激素配比,初步建立其离体培养再生体系。[结果]厚叶岩白菜愈伤组织的最佳诱导培养基为:改良MS+NAA 0.50 mg/L+6-BA 0.50 mg/L+水解酪蛋白100.00 mg/L+0.2%PVP;分化培养基为:改良MS+NAA 0.50 mg/L+IBA 0.10 mg/L+6-BA0.50 mg/L;生根培养基为:1/2 MS+IBA 0.50 mg/L+NAA 0.01 mg/L。15 d后移植幼苗的叶片由嫩绿变成深绿,30 d后移植幼苗长出新叶,叶片中心偏红,成活率达到95%,且幼苗生长健壮。[结论]通过对培养基组分、激素配比及环境因子的筛选,为野生厚叶岩白菜初步建立了一个实用的离体培养再生技术体系。[ Objective ] The research aimed to establish the complete regeneration system of Bergenia crassifolia and lay a foundation for researching its genetic transformation. [ Method ] With leaves of wild B. crassifolia as explants, its regeneration system cultured in vitro was established preliminarily through screening media and hormone proportions. [ Result] The optimum induction medium of B. crassifolia callus was improved MS ＋ NAA 0.50 mg/L ＋ 6-BA 0.50 mg/L ＋ dried hydrolyzed casein 100.00 mg/L ＋ 0.2% PVP. Its differentiation medium was improved MS ＋ NAA 0.50 mg/L ＋ IBA 0.10 mg/L ＋ 6-BA 0.50 mg/L. Its rooting medium was 1/2 MS ＋ IBA 0.50 mg/L ＋ NAA 0.01 mg/L. The leaves of transplanted seedlings became dark green from yellow green after 15 d, the new leaves grew out from transplanted seedlings after 30 d and the centers of the leaves were slight red. The survival rate of transplanted seedlings reached 95% and they grew healthily. [ Conclusion ] A practical technology system for in vitro culture and regeneration of wild B. crassifolia was established preliminarily through screening medium components, hormone proportions and environmental factors.

关 键 词：野生厚叶岩白菜 叶片外植体 组织培养 愈伤组织 离体再生 

分 类 号：S603.6[农业科学—园艺学]