基于改进CTAB法提取空间诱变柱花草总DNA研究  

Extraction of DNA from the Space Mutation in Stylo by Improved CTAB Method

作　　者：严琳玲[1,2] 赵琼玲[1,2] 白昌军[2] 刘国道[2] 

机构地区：[1]海南大学儋州校区农学院,海南儋州571737 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州573717

出　　处：《安徽农业科学》2009年第8期3465-3467,共3页Journal of Anhui Agricultural Sciences

基　　金：农业部"973"项目(2007CB108903)

摘　　要：[目的]用改进CTAB法提取空间诱变柱花草的总DNA。[方法]以柱花草幼苗叶片为材料，采用改良CTAB法提取柱花草总DNA。[结果]所提的DNA凝胶条带清晰，无拖尾现象，浓度在406．6～857．3ng／μl，0D260／DD230检测值在1．96—2．18，OD260／0D280检测值在1．76—1．98。所提DNA适用于ISSR-PCR。[结论]该方法具有简便、快速、高效的特点。[ Objective ] The research aimed to extract the genomie DNA of Stylosanthes spp. by the improved method of CTAB-DNA isolation. [ Method] The improved method of CTAB-DNA isolation was used to extract genomic DNA from the seedling leaves of the space mutation in Stylosanthes spp, [ Result] High quality total DNA was isolated, and the concentration was 406.6 -857.3 ng/μl. The values of OD260/OD230 of each sample were between 1.96 and 2.18, the values of OD260/OD280 were between 1.76 and 1.98. The DNA extracted by CTAB can be used for ISSR-PCR. [ Conclusion] The improved method of CTAB-DNA isolation had characteristics of simple, rapid and effective.

关 键 词：柱花草 空间诱变 CTAB DNA ISSR—PCR 

分 类 号：S542.9[农业科学—作物学]