提高麻疹乙脑二联活疫苗热稳定性的研究  

Improvement of Heat Stability of Live Measles-Japanese Encephalitis Vaccine

作　　者：姚亚夫[1] 张卓光 王洪彬[1] 周蓉 李玉华[1] 史东惠 彭维 张大全 何建琴 张艳[1] 王寿贵[1] 

机构地区：[1]卫生部成都生物制品研究所,成都610063

出　　处：《中国生物制品学杂志》1998年第1期25-27,共3页Chinese Journal of Biologicals

摘　　要：首先对3种保护剂冻干的麻疹乙脑二联苗进行滴度比较，结果3种保护剂的二联疫苗中麻疹病毒滴度差异均无显著意义；而对乙脑病毒滴度显示出保护剂Ⅱ和Ⅲ明显优于Ⅰ。选择保护剂Ⅲ制备二联苗和单价苗进行热稳定性试验，其中麻疹病毒在37℃对天及50℃72h的滴度下降均＜1．0Log。乙脑病毒在37℃14天及在50℃24h滴度下降均＜1．0Log。表明保护剂Ⅲ对麻疹沪191株和乙脑14－2株病毒的热保护作用均好。The titers of live measles-Japanese encephalitis (JE) vaccines using 3 kinds ofprotective agents were compared. The resu1t showed no significant difference among themeasles virus titers of them. However, the JE virus titers of the vaccines using protectiveagents Ⅱ and Ⅲ were significantly higher than that using protective agent Ⅰ. Theheat stabilities of monovalent measles, JE and bivalent measles-JE vaccines using protective agent Ⅲwere studied. The titer of measles virus decreased by <1. 0Log after being stored at 37℃ for21 days or 50℃ for 72h, and that of JE virus decreased by <1.0Log after being stored at37℃ for 14 days or 50℃ for 24h. The results showed good protection of agent Ⅲ to Hu191strain of measles virus and SA14-14-2 strain of JE virus.

关 键 词：麻疹 乙脑 二联活疫苗 保护剂 热稳定性 

分 类 号：R511.101[医药卫生—内科学] R512.320.1[医药卫生—临床医学]