大麦与纤毛鹅观草属间杂种F_5和BC_1F_4的同工酶研究  

ISOZYME ANALYSIS OF F5 AND BC1F4 FROM CULTIVATED BARLEY (HORDEUM VULGARE)×ROEGNERIA CILIARIS

作　　者：李万几[1] 李逸平[1] 刘芳[1] 

机构地区：[1]四川农业大学小麦研究所

出　　处：《Acta Botanica Sinica》1998年第3期263-269,共7页Acta Botanica Sinica（植物学报：英文版）

摘　　要：对栽培大麦（ＨｏｒｄｅｕｍｖｕｌｇａｒｅＬ．品种“Ａｒｕｐｏ”）与纤毛鹅观草（Ｒｏｅｇｎｅｒｉａｃｉｌｉａｒｉｓ（Ｔｒｉｎ．）Ｎｅｖｓｋｉ）属间杂种后代Ｆ５和回交后代ＢＣ１Ｆ４（以“Ａｒｕｐｏ”作回交亲本）的不同类型株系与其双亲的幼根、幼芽、幼穗、幼嫩籽粒分别进行了酯酶、过氧化物酶同工酶分析。结果Ｆ５和ＢＣ１Ｆ４各株系的酯酶同工酶、过氧化物酶同工酶酶谱具有母本“Ａｒｕｐｏ”全部或绝大多数酶带，父本Ｒ．ｃｉｌｉａｒｉｓ的１～３条酶带和数量不等的双亲所没有的新酶带，也出现酶带的减少。结果表明，纤毛鹅观草的遗传物质已稳定地遗传给了其自交和回交后代。酶谱变异与植株习性出现一定程度的关联。由于在不同时期内结构基因的表达起不同作用，因此，在用同工酶分析法鉴定易位系时，最好不同的器官采用不同的酶类或一种同工酶多时期连续分析。Esterase and peroxidase isozymes were analysed in the variants including 4 types, 16 lines of F5, BC1F4 and the parents derived from cultivated barley (Hordeum vulgare cv. Arupo)×Roegneria ciliaris (Trin.) Nevski in young roots, shoots, spikes and seeds. The zymogram patterns of esterase and peroxidase demonstrated that the 16 lines of F5 and BC1F4 had all or most bands of the cultivated barley parent cv. “Arupo”, 1 to 3 bands from the male R. ciliaris, and new hybrid isozyme bands in various amount. Some bands of parent “Arupo” were lost. It suggested that the genetic substances come from R. ciliaris were stably inherited to the progenies of selfing and backcrossing, and there were some variations among the lines. There was certain relationship between isozyme variance and plant characters. Thus,in identifying the translocation lines by isozyme analysis, it would be preferable to study the various organspecific isozymes or to trace one type of isozyme pattern in consequence.

关 键 词：栽培大麦 纤毛鹅观草 属间杂种 酯酶 过氧化物酶 

分 类 号：Q943[生物学—植物学] S512.303.2[农业科学—作物学]