重组轮状病毒SA11 Vp4抗原表位诱导广泛交叉中和抗体反应  

Broadly Immunological Reativities Primed by Epitopes Corresponding to the Cleavage Region of SA11 Vp

作　　者：陈元鼎[1] 刘名英[1] 赵玮[1] 戴长伯 

机构地区：[1]中国协和医科大学医学生物学研究所

出　　处：《中国病毒学》1998年第1期57-63,共7页Virologica Sinica

基　　金：云南省科委研究基金

摘　　要：本文报导在ＲＨＶＲＮＡ２载体系统中表达的轮状病毒（ＲＶ）Ｖｐ４胰酶切割位点区抗原表位可诱导动物产生具有广泛交叉中和反应的血清抗体。携带有抗原表位ＲＥＡ、ＲＥＢ和ＲＥＣ，及其组合ＲＥＡ＋ＲＥＢ＋ＲＥＣ基因的ｐＥＴ重组质粒，高水平表达了这些与载体蛋白嵌合的抗原表位。进一步研究结果表明，这些抗原表位所诱导的动物血清抗体，具有广泛识别同源及异源血清型ＲＶ抗原的能力，和体外中和这些同源及异源血清型ＲＶ病毒感染性的能力。研究结果揭示ＲＶ胰酶切割位点区氨基酸序列具有重要的免疫学功能，它在ＦＨＶＲＮＡ２外源抗原表位载体系统中表达后，可作为新一代ＲＶ抗原表位亚单位疫苗的候选疫苗抗原。With the flock house virus (FHV) capsid protein as a vector (FHV RNA 2),the epitopes corresponding to the cleavage regions of rotavirus SA11 Vp4 primed broadly cross reactive neutralizing antibodies in guinea pigs. The epitopes of SA11 Vp4:REA(223～242aa),REB(243～262aa),REC(234～251aa),and their combination REA+REB+REC were expressed on the chimeric protein surface in the recombinant pET FHV RE system,and used to immunize guinea pigs to prepare antisera. The antisera were proven to have strong cross recognizing and cross neutralizing activities to homologous SA11 virus and heterologous Wa,S2,DS 1,Ito Sty 3, St. Thomase, Gottfried, UK, and B223 virus strains. The results suggested that the amino acid sequences of rotavirus Vp4 cleavage region have important immunological functions,and when displayed in the proper positions on the FHV RNA 2 protein vector, these epitopes could be prospective as a new type of epitope based subunit vaccines.

关 键 词：轮状病毒 胰酶切割位点 交叉反应 疫苗 

分 类 号：R373.2[医药卫生—病原生物学] R392－33[医药卫生—基础医学]