PCR-SSP分型检测上海汉族人群HLA-Cw位点血清学和非血清学鉴定的等位基因  

HLACw Genotyping of Serologically and Nonserologically DefinedAlleles Using Sequencespecific Primers(PCRSSP)in a Shanghai Han Population

作　　者：冯明亮[1] 张雁征[1] 杨颖[1] 陆琼[1] 冯哲[1] 陈仁彪[2] 

机构地区：[1]上海市血液中心上海市输血研究所 [2]上海第二医科大学

出　　处：《Acta Genetica Sinica》1998年第2期95-102,共8页

摘　　要：应用补体介导的微量淋巴细胞毒实验可检出ＨＬＡＣｗ１～１０，但有２０％～５０％的空白（ｂｌａｎｋ）不能检出。利用ＰＣＲＳＳＰ对７０例上海汉族样本进行ＨＬＡＣｗ等位基因分型，首次在中国汉族人群中检出Ｃｗ１２０１／１２０２、１２０３、１３０１、１４、１６０１、１６０２、１７０１等非血清学鉴定的抗原，并对血清学鉴定抗原的基因进行分型，依次为Ｃｗ０１、０２、０３０２／０３０４、０３０３、０４、０５０１、０６０２、０７０１／０７０２／０７０３及０８。ＰＣＲＳＰ分型使上海汉族人群ＨＬＡＣｗ位点空白基因频率从０．３０７下降到０．０２８。１２例样本检出Ｃｗ０８，与原来认为中国人群中Ｃｗ８罕见的观点不符。结果证明，ＰＣＲＳＳＰ用于ＨＬＡＣｗ基因分型是快速、准确的。? The new classical complementmediated microlymphocytotoxicity test can detect a total of 10 different serologically defined antigen specificities(Cw1～10)encoded by HLACw locus. However, the blank amounts to 20%～50% for there are Cw antigens which can not be detected with antisera available. We adopted a PCRSSP method to genotype a sample of 70 subjects collected from a Chinese Han population in Shanghai.We identified for the first time the following nonserologically defined HLACw alleles in the Chinese Han population: Cw*1201/1202,1203,1301,14,1601,1602,1701, and also the serologically defined HLACw alleles such as: Cw*01,02,0302/0304,0303,04,0501,0602,0701/0702/0703,08. With this sample we have made a survey of HLACw allele frequencies in a Chinese Han population in Shanghai, with blank frequency being lowered from 0.307 to 0.028. The Cw*08 was originally considered to be rare in Chinese population. However, we found 12 cases of Cw*08 in this sample, which clearly does not coincide with the original point of view. Our results comfirmed that PCRSSP typing is rapid and accurate for HLACw alleles.

关 键 词：HLA-CW PCR 血清学 鉴定抗原 非血清学 汉族 

分 类 号：Q347[生物学—遗传学]