大鼠E12.5胰腺间质细胞原代培养及鉴定  

Primary culture and characterization of Mesenchymal cells of E12.5 rat pancreas

作　　者：仲新村[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学与分子生物学系,江苏南京210029

出　　处：《现代生物医学进展》2009年第6期1071-1072,1075,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30670781)

摘　　要：目的:体外分离培养大鼠胚胎胰腺间质细胞(pancreas mesenchymal cells)。方法:以孕12.5天(E12.5)的大鼠胚胎为组织来源,通过显微分离得到E12.5的胚胎胰腺,在鼠尾胶原包覆的六孔板内进行组织块培养,观察培养过程中胰芽的形态变化,间质细胞的长出,并用细胞免疫荧光的方法对间质细胞进行了鉴定。结果:显微分离得到了胚胎胰腺,为间质细胞群包绕着上皮细胞团的结构,培养第二天即发现间质细胞的长出,培养第四天时得到间质细胞群,免疫荧光鉴定为vimentin阳性的间质细胞。结论:本方法通过大鼠E12.5胚胎胰腺显微分离,体外培养出原代胚胎胰腺间质细胞,为进一步研究大鼠胰腺间质细胞的功能提供实验平台。Objective： Isolation and culture of E12.5 rat pancreas mesenchymal cells. Methods： The pancreas was dissected by micro-dissection from the E12.5 rat embryo, then cultured in rat tail collagen coated 6-well plate. The morphologic change and mesenchymal cells＇ outgrowth were detected, and the cell type was characterized by cell immunofluorescence. Results： The embryonic pancreas was successfully dissected. They appeared to be a construction of epithelial cells surrounded by mesenchymal cells. The mesenchymal cells began outgrowing when cultured for 2 days and formed mesenchymal cell mass after 4 days＇ culture. The cell mass was characterized by immunofluorescence of vimentin, a mesenchymal cell marker. Conclusions： In this study, the E12.5 pancreata have been successfully dissected and the primary mesenchymal cells were cultured, which can provide a good platform for the study on the function of the mesenchymalcells.

关 键 词：胚胎胰腺 原代培养 间充质细胞 免疫荧光 

分 类 号：R587.1[医药卫生—内分泌]