脊髓灰质炎病毒重组体的构建及其抗原性状分析  

THE CONSTRUCTION OF POLIOVIRUS CHIMERA AND THE ANALYSIS OF ITS ANTIGENITIC STRUCTURE

作　　者：李琦涵[1] 姜莉[1] 昝云红[1] 赵红玲 郭仁[1] 

机构地区：[1]中国医学科学院

出　　处：《微生物学报》1998年第2期86-91,共6页Acta Microbiologica Sinica

基　　金：云南省应用基础研究基金!92C115

摘　　要：以脊髓灰质炎病毒（以下简称脊灰病毒）为载体构建的重组体活病毒可以做为探讨脊灰病毒的抗原结构和特性的有益手段。在构建重组有甲型肝炎病毒小片段抗原多肽的重组脊灰病毒基础上，分析了脊灰病毒VP1上中和抗原位点I的空间构象特点，并探讨了插入的外源抗原片段对其空间结构的可能影响。The construction of poliovirus chimera is an useful way to inveshgate the antigenic structure conformahon of poliovirus. In this research, the poliovirus chimera containing a piece of antigenic fragment of hepatitis A virus was constructed for analysis of poliovirus antigenic structure conformation. Depending on the characterization of this chimera, the conformation of poliovirus neutralization antigenic site I in VP1 was analyzed, and that the possible influence of hepatitis A virus antigenic fragment inserted to the structure conformation of poliovirus antigen was also investigated.

关 键 词：脊髓灰质炎病毒 重组体 抗原空间构象 

分 类 号：R373.22[医药卫生—病原生物学] Q784[医药卫生—基础医学]