巨大芽孢杆菌的青霉素酰化酶基因在大肠杆菌中的克隆和表达(英文)  

Cloning and Expression of the pga Gene of Bacillus megaterium in E.coli

作　　者：黄艳红[1] 张颖[1] 储瑞蔼[1] 吴祥甫[1] 王应睐[1] 袁中一[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1998年第2期107-113,共7页

摘　　要：从巨大芽孢杆菌（Ｂａｃｉｌｕｓｍｅｇａｔｅｒｉｕｍ）ＣＡ４０９８的基因组中，通过ＰＣＲ方法扩增青霉素酰化酶（ｐｇａ）基因，克隆到ｐＫＫ２２３－３质粒中，在Ｅ．ｃｏｌｉＨＢ１０１中得到表达。同时，测定了ｐｇａ基因的全部序列，推出氨基酸序列，再与不同菌种来源的青霉素酰化酶的氨基酸序列进行比较，表明它们的序列有一定的保守性，尤其是活性部位的保守性更强。The pga gene was amplified by PCR from Bacillus megaterium CA4098, cloned into pKK2233 vector and expressed in E.coli HB101. The pga sequence was also determined . The deduced amino acids sequence was compared with those of PGAs from other bacteria and showed that they were similar, especially the active sites were strongly conserved.

关 键 词：青霉素酰化酶 巨大芽孢杆菌 克隆 核苷酸序列 

分 类 号：Q555.3[生物学—生物化学]