核因子增强雌激素受体与雌激素效应元件的结合  

Nuclear Factors Enhance the Binding of Estrogen Receptor to Estrogen Response Element

作　　者：朱国璋[1] 杨根焰[1] 龚岳亭 张永莲[1] 

机构地区：[1]中国科学院上海生物化学研究所分子生物学国家重点实验室

出　　处：《生物化学与生物物理学报》1998年第2期198-202,共5页

基　　金：国家自然科学基金

摘　　要：利用体外翻译系统，翻译了能与雌激素效应元件（ＥＲＥ）结合的全长的人雌激素受体（ｈＥＲ）。制备了切除卵巢的雌性大鼠子宫核抽提物，在雌激素存在下，此核抽提物能增强ｈＥＲ与ＥＲＥ的结合。此核抽提物在５０℃保温１５ｍｉｎ后，明显减弱了增强ｈＥＲ－ＥＲＥ结合的作用。提示了核抽提物中存在着能增强ｈＥＲ－ＥＲＥ结合的雌激素依赖的热敏感性的辅助因子。在大肠杆菌中表达了谷胱甘肽转硫酶（ＧＳＴ）融合的雌激素受体的ＤＮＡ结合区（ＥＲＤＢＤ），表达产物（ＧＳＴ－ＥＲＤＢＤ）也能与ＥＲＥ结合，但结合不受核抽提物影响。提示了核因子增强ｈＥＲ－ＥＲＥ结合的作用很可能不是通过ＤＢＤ的区域起作用的。We obtained the full length human estrogen receptor(hER) through the in vitro translation. It was shown that the translational product could bind to the estrogen response element(ERE). The nuclear extract prepared from the rat uterus after ovariectomy could enhance the binding of hERERE in an estrogen dependent manner. However, the enhancing effect was sharply decreased when the nuclear extract was preincubated at 50 ℃ for 15 minutes before being used for the binding reaction. These results indicated the presence in the rat uterus extracts after ovariectomy of a heat labile factor which can enhance the binding of hERERE in an estrogendependent manner. The DNA binding domain of estrogen receptor(ER DBD) fused to the Scistosoma japonicam glutathione Stransferase(GST) was expressed in the E.coli. The expression product also could bind the ERE. However, the binding was not affected by the uterine extract, indicating that the heatsensitive nuclear factors may interact with the hER outside the DBD to enhance the binding of ER to ERE.

关 键 词：核因子 雌激素 受体 效应元件 

分 类 号：Q579.13[生物学—生物化学]