甲氨蝶呤对小鼠抗早孕作用的研究  

EFFECTS OF METHOTREXATE ON EARLY PREGNANCY IN MICE

作　　者：王乃功[1,2] 关慕贞 郑卫红[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所 [2]湖北三峡学院医学院

出　　处：《药学学报》1998年第1期10-13,共4页Acta Pharmaceutica Sinica

摘　　要：用小鼠抗早孕实验，观察甲氨蝶呤（ＭＴＸ）单用和合并用药的抗早孕作用。小鼠于妊娠ｄ７分别一次ｓｃＭＴＸ８，２４和７２ｍｇ·ｋｇ－１可引起胚胎死亡，妊娠ｄ１３死亡率分别为１８０％，５５５％和６０３％。给药后测定血清孕酮水平，妊娠ｄ９未发现明显改变，妊娠ｄ１３其水平明显降低。小鼠于妊娠ｄ７～９注射ＭＴＸ２４ｍｇ·ｋｇ－１连续３ｄ引起胚胎全部死亡。ｈＣＧ和孕酮可拮抗ＭＴＸ的抗早孕作用。ＲＵ４８６可增强ＭＴＸ的抗早孕作用，提示两药合用抗早孕有协同作用。Single sc injection of MTX at dosages of 8, 24 and 72 mg·kg-1 on day 7 of gestation was found to have a feticide effect in mice. The mortality of fetuses on day 13 of pregnancy was 180%, 555% and 603%,　respectively. Treatment with MTX caused a decrease of serum progesterone level on day 13 of pregnancy. Treatment with MTX at a dosage of 24 mg·kg-1 on day 7～9 of gestation terminated total early pregnancy. MTX combined with hCG 10 U/mouse or pregesterone 02 mg/mouse was shown to have antagonistic effect to terminate early pregnancy. Synergism of RU486 combined with MTX on termination of early pregnancy in mice was observed.

关 键 词：甲氨蝶呤 孕酮 抗早孕 

分 类 号：R979.21[医药卫生—药品]