雷公藤红素对大鼠血管平滑肌细胞c-myc和血小板源性生长因子的mRNA的影响  

Effect of Tripterine on mRNA Expression of c myc and Platelet Derived Growth Factor of Vascular Smooth Muscle Cell in Rats

作　　者：陈星[1] 汪洛[2] 丰美福[2] 朱国英[1] 

机构地区：[1]北京医科大学第一医院,北京100034 [2]中国科学院动物研究所生物膜与膜生物工程国家重点实验室

出　　处：《中国中西医结合杂志》1998年第3期156-158,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：卫生部科研基金资助课题!(No.94- 1- 2 48)

摘　　要：目的 :观察雷公藤红素对大鼠血管平滑肌细胞 (VSMC)中原癌基因 (c myc)和血小板源性生长因子 (PDGF)的mRNA表达水平的影响。方法 :分别将所培养的第 5～ 1 0代VSMC同步化培养 2 4h后 ,加入 2 0 %胎牛血清 (FCS)和不同剂量的雷公藤红素 (0 1、0 2和0 3mg/L) ,共同孵育 6h和 1 2h后 ,分别提取VSMC胞浆总RNA ,通过地高辛标记探针 ,采用斑点杂交方法检测c myc和PDGF的mRNA表达。 结果 :经雷公藤红素作用后 ,VSMC的c myc和PDGF的mRNA表达较对照组明显降低 ,c mycmRNA表达水平的降低具有一定的药物剂量依赖性关系。结论 :雷公藤红素可能是通过抑制VSMC的c myc和PDGF的mRNA的表达 ,而引起抑制VSMC的过度增殖。Objective: To observe the effect of Tripterine on mRNA expression of c myc and platelet derived growth factor (PDGF) in vascular smooth muscle cell (VSMC) of rats. Methods: 5～10th passage culture of VSMC was used. Tripterine and 20% fetal calf serum were added into the medium of cultured VSMC in concentration of 0 1,0 2 and 0 3 mg/L after free serum cultivation for 24 hours. The general RNA was isolated from VSMC at 6 and 12 hours after the drug addition for detection of mRNA expression of c myc and PDGF respectively by dot blot hybridization. The cDNA probes were labeled by digoxin. Results: Tripterine inhibited the PDGF mRNA expression of VSMC and decreased c myc mRNA expression in a dose dependent manner, either vs. control. Conclusion: Tripterine could inhibit mRNA expression of c myc and PDGF in VSMC, therefore, it would inhibit overproliferation of VSMC.

关 键 词：雷公藤红素 血管平滑肌 血小板源性 生长因子 

分 类 号：R285.5[医药卫生—中药学]